零件制造者:查找、生成和分析生物零件。

IF 2.6 Q2 BIOCHEMICAL RESEARCH METHODS Synthetic biology (Oxford, England) Pub Date : 2019-06-04 eCollection Date: 2019-01-01 DOI:10.1093/synbio/ysz014
Emily Scher, Shay B Cohen, Guido Sanguinetti
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引用次数: 1

摘要

合成生物学领域在实践上和哲学上都依赖于生物艺术的理念——具体的DNA序列意味着离散的功能。虽然有许多软件工具允许用户通过将生物零件或遗传特征拼接到遗传设备中来设计复杂的DNA序列,但缺乏帮助合成生物学家寻找生物零件和生成新零件的工具。在实践中,研究人员经常以一种特殊的方式找到生物零件。我们提出了PartCrafter,一个提取和汇总基因组特征数据的工具,以促进具有特定功能的新生物零件的搜索。PartCrafter还可以根据任何制造标准对基因组特征进行包装,为新宿主优化密码子,并去除禁止的位点,从而将基因组特征转化为生物艺术。partcraft可以在partcrafter.com上找到。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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PartCrafter: find, generate and analyze BioParts.

The field of Synthetic Biology is both practically and philosophically reliant on the idea of BioParts-concrete DNA sequences meant to represent discrete functionalities. While there are a number of software tools which allow users to design complex DNA sequences by stitching together BioParts or genetic features into genetic devices, there is a lack of tools assisting Synthetic Biologists in finding BioParts and in generating new ones. In practice, researchers often find BioParts in an ad hoc way. We present PartCrafter, a tool which extracts and aggregates genomic feature data in order to facilitate the search for new BioParts with specific functionalities. PartCrafter can also turn a genomic feature into a BioPart by packaging it according to any manufacturing standard, codon optimizing it for a new host, and removing forbidden sites. PartCrafter is available at partcrafter.com.

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