Xiangcheng Zhan, Jingwei Zhang, Saiyang Li, Xiaolu Zhang, Linchao Li, Tiantian Song, Qunlong Liu, Jun Lu, Yunfei Xu, Ren-Shan Ge
{"title":"单核细胞趋化蛋白-1刺激大鼠干细胞和祖间质细胞在再生过程中的分化。","authors":"Xiangcheng Zhan, Jingwei Zhang, Saiyang Li, Xiaolu Zhang, Linchao Li, Tiantian Song, Qunlong Liu, Jun Lu, Yunfei Xu, Ren-Shan Ge","doi":"10.1186/s12861-020-00225-1","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Monocyte chemoattractant protein-1(MCP-1) is a chemokine secreted by Leydig cells and peritubular myoid cells in the rat testis. Its role in regulating the development of Leydig cells via autocrine and paracrine is still unclear. The objective of the current study was to investigate the effects of MCP-1 on Leydig cell regeneration from stem cells in vivo and on Leydig cell development in vitro.</p><p><strong>Results: </strong>Intratesticular injection of MCP-1(10 ng/testis) into Leydig cell-depleted rat testis from post-EDS day 14 to 28 significantly increased serum testosterone and luteinizing hormone levels, up-regulated the expression of Leydig cell proteins, LHCGR, SCARB1, CYP11A1, HSD3B1, CYP17A1, and HSD17B3 without affecting progenitor Leydig cell proliferation, as well as increased ERK1/2 phosphorylation. MCP-1 (100 ng/ml) significantly increased medium testosterone levels and up-regulated LHCGR, CYP11A1, and HSD3B1 expression without affecting EdU incorporation into stem cells after in vitro culture for 7 days. RS102895, a CCR2 inhibitor, reversed MCP-1-mediated increase of testosterone level after culture in combination with MCP-1.</p><p><strong>Conclusion: </strong>MCP-1 stimulates the differentiation of stem and progenitor Leydig cells without affecting their proliferation.</p>","PeriodicalId":9130,"journal":{"name":"BMC Developmental Biology","volume":"20 1","pages":"20"},"PeriodicalIF":0.0000,"publicationDate":"2020-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s12861-020-00225-1","citationCount":"3","resultStr":"{\"title\":\"Monocyte Chemoattractant Protein-1 stimulates the differentiation of rat stem and progenitor Leydig cells during regeneration.\",\"authors\":\"Xiangcheng Zhan, Jingwei Zhang, Saiyang Li, Xiaolu Zhang, Linchao Li, Tiantian Song, Qunlong Liu, Jun Lu, Yunfei Xu, Ren-Shan Ge\",\"doi\":\"10.1186/s12861-020-00225-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Monocyte chemoattractant protein-1(MCP-1) is a chemokine secreted by Leydig cells and peritubular myoid cells in the rat testis. Its role in regulating the development of Leydig cells via autocrine and paracrine is still unclear. The objective of the current study was to investigate the effects of MCP-1 on Leydig cell regeneration from stem cells in vivo and on Leydig cell development in vitro.</p><p><strong>Results: </strong>Intratesticular injection of MCP-1(10 ng/testis) into Leydig cell-depleted rat testis from post-EDS day 14 to 28 significantly increased serum testosterone and luteinizing hormone levels, up-regulated the expression of Leydig cell proteins, LHCGR, SCARB1, CYP11A1, HSD3B1, CYP17A1, and HSD17B3 without affecting progenitor Leydig cell proliferation, as well as increased ERK1/2 phosphorylation. MCP-1 (100 ng/ml) significantly increased medium testosterone levels and up-regulated LHCGR, CYP11A1, and HSD3B1 expression without affecting EdU incorporation into stem cells after in vitro culture for 7 days. RS102895, a CCR2 inhibitor, reversed MCP-1-mediated increase of testosterone level after culture in combination with MCP-1.</p><p><strong>Conclusion: </strong>MCP-1 stimulates the differentiation of stem and progenitor Leydig cells without affecting their proliferation.</p>\",\"PeriodicalId\":9130,\"journal\":{\"name\":\"BMC Developmental Biology\",\"volume\":\"20 1\",\"pages\":\"20\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-10-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1186/s12861-020-00225-1\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BMC Developmental Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1186/s12861-020-00225-1\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Developmental Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s12861-020-00225-1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
Monocyte Chemoattractant Protein-1 stimulates the differentiation of rat stem and progenitor Leydig cells during regeneration.
Background: Monocyte chemoattractant protein-1(MCP-1) is a chemokine secreted by Leydig cells and peritubular myoid cells in the rat testis. Its role in regulating the development of Leydig cells via autocrine and paracrine is still unclear. The objective of the current study was to investigate the effects of MCP-1 on Leydig cell regeneration from stem cells in vivo and on Leydig cell development in vitro.
Results: Intratesticular injection of MCP-1(10 ng/testis) into Leydig cell-depleted rat testis from post-EDS day 14 to 28 significantly increased serum testosterone and luteinizing hormone levels, up-regulated the expression of Leydig cell proteins, LHCGR, SCARB1, CYP11A1, HSD3B1, CYP17A1, and HSD17B3 without affecting progenitor Leydig cell proliferation, as well as increased ERK1/2 phosphorylation. MCP-1 (100 ng/ml) significantly increased medium testosterone levels and up-regulated LHCGR, CYP11A1, and HSD3B1 expression without affecting EdU incorporation into stem cells after in vitro culture for 7 days. RS102895, a CCR2 inhibitor, reversed MCP-1-mediated increase of testosterone level after culture in combination with MCP-1.
Conclusion: MCP-1 stimulates the differentiation of stem and progenitor Leydig cells without affecting their proliferation.
期刊介绍：
BMC Developmental Biology is an open access, peer-reviewed journal that considers articles on the development, growth, differentiation and regeneration of multicellular organisms, including molecular, cellular, tissue, organ and whole organism research.
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