淡水涡虫的实验室维护和繁殖

Current Protocols in Microbiology Pub Date : 2020-10-15 DOI:10.1002/cpmc.120

Makayla R. P. Dean, Elizabeth M. Duncan

{"title":"淡水涡虫的实验室维护和繁殖","authors":"Makayla R. P. Dean, Elizabeth M. Duncan","doi":"10.1002/cpmc.120","DOIUrl":null,"url":null,"abstract":"Freshwater planarians are a powerful model organism for the study of animal regeneration, stem cell maintenance and differentiation, and the development and functions of several highly conserved complex tissues. At the same time, planarians are easy to maintain, inexpensive to propagate, and reasonably macroscopic (1 mm to 1 cm in length), making them excellent organisms to use in both complex academic research and hands-on teaching laboratories. Here, we provide a detailed description of how to maintain and propagate these incredibly versatile animals in any basic laboratory setting. © 2020 Wiley Periodicals LLC.Basic Protocol 1: Salt solution preparationBasic Protocol 2: Cleaning planarian housingBasic Protocol 3: Food preparationBasic Protocol 4: Feeding planariansBasic Protocol 5: Expansion and amplification of colony","PeriodicalId":39967,"journal":{"name":"Current Protocols in Microbiology","volume":"59 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmc.120","citationCount":"0","resultStr":"{\"title\":\"Laboratory Maintenance and Propagation of Freshwater Planarians\",\"authors\":\"Makayla R. P. Dean, Elizabeth M. Duncan\",\"doi\":\"10.1002/cpmc.120\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Freshwater planarians are a powerful model organism for the study of animal regeneration, stem cell maintenance and differentiation, and the development and functions of several highly conserved complex tissues. At the same time, planarians are easy to maintain, inexpensive to propagate, and reasonably macroscopic (1 mm to 1 cm in length), making them excellent organisms to use in both complex academic research and hands-on teaching laboratories. Here, we provide a detailed description of how to maintain and propagate these incredibly versatile animals in any basic laboratory setting. © 2020 Wiley Periodicals LLC.Basic Protocol 1: Salt solution preparationBasic Protocol 2: Cleaning planarian housingBasic Protocol 3: Food preparationBasic Protocol 4: Feeding planariansBasic Protocol 5: Expansion and amplification of colony\",\"PeriodicalId\":39967,\"journal\":{\"name\":\"Current Protocols in Microbiology\",\"volume\":\"59 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-10-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/cpmc.120\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Protocols in Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/cpmc.120\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpmc.120","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

摘要

淡水涡虫是研究动物再生、干细胞维持和分化以及一些高度保守的复杂组织的发育和功能的强有力的模式生物。同时，涡虫易于饲养，繁殖成本低廉，并且相当宏观(长度为1毫米至1厘米)，使它们成为复杂学术研究和动手教学实验室的优秀生物。在这里，我们提供了如何在任何基本的实验室环境中维持和繁殖这些令人难以置信的多功能动物的详细描述。©2020 Wiley期刊有限公司基本方案1:盐溶液制备基本方案2:清洁涡虫外壳基本方案3:食物制备基本方案4:喂养涡虫基本方案5:菌落的扩展和扩增

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

Laboratory Maintenance and Propagation of Freshwater Planarians

Freshwater planarians are a powerful model organism for the study of animal regeneration, stem cell maintenance and differentiation, and the development and functions of several highly conserved complex tissues. At the same time, planarians are easy to maintain, inexpensive to propagate, and reasonably macroscopic (1 mm to 1 cm in length), making them excellent organisms to use in both complex academic research and hands-on teaching laboratories. Here, we provide a detailed description of how to maintain and propagate these incredibly versatile animals in any basic laboratory setting. © 2020 Wiley Periodicals LLC.

Basic Protocol 1: Salt solution preparation

Basic Protocol 2: Cleaning planarian housing

Basic Protocol 3: Food preparation

Basic Protocol 4: Feeding planarians

Basic Protocol 5: Expansion and amplification of colony

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Current Protocols in Microbiology Immunology and Microbiology-Parasitology

自引率

0.00%

发文量

期刊介绍： Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.

期刊最新文献

Psychological flexibility, temperament, and perceived stress. Issue Information Programmable Gene Knockdown in Diverse Bacteria Using Mobile-CRISPRi Gene Editing in Dimorphic Fungi Using CRISPR/Cas9 Vibrio parahaemolyticus: Basic Techniques for Growth, Genetic Manipulation, and Analysis of Virulence Factors