循环CD4+ T细胞中IL-6和IL-17A的胞浆内表达与类风湿关节炎的疾病活动密切相关并预测疾病活动:加纳的一项病例对照研究

IF 2.3 Q2 RHEUMATOLOGY International Journal of Rheumatology Pub Date : 2020-10-08 eCollection Date: 2020-01-01 DOI:10.1155/2020/2808413
Samuel Asamoah Sakyi, Tonnies Abeku Buckman, Daniel Antwi-Berko, Kwame Yeboah-Mensah, Dzifa Dey, Eddie-Williams Owiredu, Benjamin Amoani, Richard Mantey
{"title":"循环CD4+ T细胞中IL-6和IL-17A的胞浆内表达与类风湿关节炎的疾病活动密切相关并预测疾病活动:加纳的一项病例对照研究","authors":"Samuel Asamoah Sakyi,&nbsp;Tonnies Abeku Buckman,&nbsp;Daniel Antwi-Berko,&nbsp;Kwame Yeboah-Mensah,&nbsp;Dzifa Dey,&nbsp;Eddie-Williams Owiredu,&nbsp;Benjamin Amoani,&nbsp;Richard Mantey","doi":"10.1155/2020/2808413","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>T cell cytokines play important roles in the development and progression of rheumatoid arthritis (RA). Loss of Th1/Th2 and Th17/Treg balance has been reported in several inflammatory autoimmune diseases. However, their role in RA within hitherto rare Ghanaian context has not been explored. Here, we evaluated the intracytoplasmic CD4+ T cell cytokine patterns in rheumatoid arthritis patients in Ghana and determined their relationship with disease activity.</p><p><strong>Methods: </strong>This case-control study included 48 newly diagnosed RA patients and 30 apparent healthy controls from two major hospitals in Ghana. Validated structured questionnaires were administered to obtain demographic data; blood samples were collected and processed for flow cytometric analysis.</p><p><strong>Results: </strong>IFN-<i>γ</i>, TNF-<i>α</i>, IL-4, IL-6, IL-10, IL-17A, IL-6/IL-4, and IL-17/IL-10 expressions were significantly higher in RA cases compared to the healthy controls. The expression of IL-6 (0.00 (0.00-0.98) vs. 0.82 (0.34-1.10) vs. 1.56 (1.39-1.68), <i>p</i> < 0.0001), IL-17A (0.00 (0.00-0.02) vs. 0.19 (0.09-0.30) vs. 0.99 (0.64-1.25), <i>p</i> < 0.0001), and IL-17A/IL-10 (0.00 (0.00-0.39) vs. 0.15 (0.09-0.26) vs. 0.88 (0.41-1.47), <i>p</i> < 0.0001) increased significantly from the healthy controls through RA patients with low DAS scores to RA patients with moderate DAS scores. IL-6 (<i>β</i> = 0.681, <i>r</i> <sup>2</sup> = 0.527, <i>p</i> < 0.0001), IL-17A (<i>β</i> = 0.770, <i>r</i> <sup>2</sup> = 0.593, <i>p</i> < 0.0001), and IL-17A/IL-10 (<i>β</i> = 0.677, <i>r</i> <sup>2</sup> = 0.452, <i>p</i> < 0.0001) expressions were significantly directly associated with DAS28 scores. IL-6 (cutoff = 1.32, sensitivity = 100.0%, specificity = 100.0%, accuracy = 100.0%, and AUC = 1.000) and IL-17A (cutoff = 0.58, sensitivity = 100.0%, specificity = 100.0%, accuracy = 100.0%, and AUC = 1.000) presented with the best discriminatory power in predicting moderate DAS scores from low DAS scores.</p><p><strong>Conclusion: </strong>Th1- and Th17-related cytokines predominate in the pathophysiology of RA, with IL-6 and IL-17 being principally and differentially expressed based on the severity of the disease. IL-6 and IL-17A could serve as useful prognostic and disease-monitoring markers in RA in the African context.</p>","PeriodicalId":51715,"journal":{"name":"International Journal of Rheumatology","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2020-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/2808413","citationCount":"6","resultStr":"{\"title\":\"Intracytoplasmic Expression of IL-6 and IL-17A in Circulating CD4+ T Cells Are Strongly Associated with and Predict Disease Activity in Rheumatoid Arthritis: A Case-Control Study in Ghana.\",\"authors\":\"Samuel Asamoah Sakyi,&nbsp;Tonnies Abeku Buckman,&nbsp;Daniel Antwi-Berko,&nbsp;Kwame Yeboah-Mensah,&nbsp;Dzifa Dey,&nbsp;Eddie-Williams Owiredu,&nbsp;Benjamin Amoani,&nbsp;Richard Mantey\",\"doi\":\"10.1155/2020/2808413\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>T cell cytokines play important roles in the development and progression of rheumatoid arthritis (RA). Loss of Th1/Th2 and Th17/Treg balance has been reported in several inflammatory autoimmune diseases. However, their role in RA within hitherto rare Ghanaian context has not been explored. Here, we evaluated the intracytoplasmic CD4+ T cell cytokine patterns in rheumatoid arthritis patients in Ghana and determined their relationship with disease activity.</p><p><strong>Methods: </strong>This case-control study included 48 newly diagnosed RA patients and 30 apparent healthy controls from two major hospitals in Ghana. Validated structured questionnaires were administered to obtain demographic data; blood samples were collected and processed for flow cytometric analysis.</p><p><strong>Results: </strong>IFN-<i>γ</i>, TNF-<i>α</i>, IL-4, IL-6, IL-10, IL-17A, IL-6/IL-4, and IL-17/IL-10 expressions were significantly higher in RA cases compared to the healthy controls. The expression of IL-6 (0.00 (0.00-0.98) vs. 0.82 (0.34-1.10) vs. 1.56 (1.39-1.68), <i>p</i> < 0.0001), IL-17A (0.00 (0.00-0.02) vs. 0.19 (0.09-0.30) vs. 0.99 (0.64-1.25), <i>p</i> < 0.0001), and IL-17A/IL-10 (0.00 (0.00-0.39) vs. 0.15 (0.09-0.26) vs. 0.88 (0.41-1.47), <i>p</i> < 0.0001) increased significantly from the healthy controls through RA patients with low DAS scores to RA patients with moderate DAS scores. IL-6 (<i>β</i> = 0.681, <i>r</i> <sup>2</sup> = 0.527, <i>p</i> < 0.0001), IL-17A (<i>β</i> = 0.770, <i>r</i> <sup>2</sup> = 0.593, <i>p</i> < 0.0001), and IL-17A/IL-10 (<i>β</i> = 0.677, <i>r</i> <sup>2</sup> = 0.452, <i>p</i> < 0.0001) expressions were significantly directly associated with DAS28 scores. IL-6 (cutoff = 1.32, sensitivity = 100.0%, specificity = 100.0%, accuracy = 100.0%, and AUC = 1.000) and IL-17A (cutoff = 0.58, sensitivity = 100.0%, specificity = 100.0%, accuracy = 100.0%, and AUC = 1.000) presented with the best discriminatory power in predicting moderate DAS scores from low DAS scores.</p><p><strong>Conclusion: </strong>Th1- and Th17-related cytokines predominate in the pathophysiology of RA, with IL-6 and IL-17 being principally and differentially expressed based on the severity of the disease. IL-6 and IL-17A could serve as useful prognostic and disease-monitoring markers in RA in the African context.</p>\",\"PeriodicalId\":51715,\"journal\":{\"name\":\"International Journal of Rheumatology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2020-10-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1155/2020/2808413\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Rheumatology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/2020/2808413\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2020/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"RHEUMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Rheumatology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2020/2808413","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2020/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"RHEUMATOLOGY","Score":null,"Total":0}
引用次数: 6

摘要

背景:T细胞因子在类风湿关节炎(RA)的发生发展中起重要作用。Th1/Th2和Th17/Treg平衡的丧失已在几种炎症性自身免疫性疾病中报道。然而,在迄今罕见的加纳背景下,它们在RA中的作用尚未得到探讨。在这里,我们评估了加纳类风湿性关节炎患者的胞浆内CD4+ T细胞细胞因子模式,并确定了它们与疾病活动性的关系。方法:本病例对照研究包括48名新诊断的RA患者和30名表面健康对照者,来自加纳两家主要医院。使用经过验证的结构化问卷来获取人口统计数据;采集血样进行流式细胞术分析。结果:RA患者中IFN-γ、TNF-α、IL-4、IL-6、IL-10、IL-17A、IL-6/IL-4、IL-17/IL-10的表达均显著高于健康对照组。IL-6 (0.00 (0.00-0.98) vs. 0.82 (0.34-1.10) vs. 1.56 (1.39-1.68), p < 0.0001)、IL-17A (0.00 (0.00-0.02) vs. 0.19 (0.09-0.30) vs. 0.99 (0.64-1.25), p < 0.0001)、IL-17A/IL-10 (0.00 (0.00-0.39) vs. 0.15 (0.09-0.26) vs. 0.88 (0.41-1.47), p < 0.0001)的表达水平从健康对照组到DAS评分较低的RA患者到DAS评分中等的RA患者均显著升高。IL-6 (β = 0.681, r 2 = 0.527, p < 0.0001)、IL-17A (β = 0.770, r 2 = 0.593, p < 0.0001)、IL-17A/IL-10 (β = 0.677, r 2 = 0.452, p < 0.0001)的表达与DAS28评分直接相关。IL-6(截止值= 1.32,灵敏度= 100.0%,特异度= 100.0%,准确度= 100.0%,AUC = 1.000)和IL-17A(截止值= 0.58,灵敏度= 100.0%,特异度= 100.0%,准确度= 100.0%,AUC = 1.000)在预测DAS中低评分方面具有最佳的区分能力。结论:Th1-和th17相关的细胞因子在RA的病理生理中起主导作用,IL-6和IL-17根据病情的严重程度主要表达,并有差异表达。在非洲地区,IL-6和IL-17A可作为有用的RA预后和疾病监测标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

摘要图片

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Intracytoplasmic Expression of IL-6 and IL-17A in Circulating CD4+ T Cells Are Strongly Associated with and Predict Disease Activity in Rheumatoid Arthritis: A Case-Control Study in Ghana.

Background: T cell cytokines play important roles in the development and progression of rheumatoid arthritis (RA). Loss of Th1/Th2 and Th17/Treg balance has been reported in several inflammatory autoimmune diseases. However, their role in RA within hitherto rare Ghanaian context has not been explored. Here, we evaluated the intracytoplasmic CD4+ T cell cytokine patterns in rheumatoid arthritis patients in Ghana and determined their relationship with disease activity.

Methods: This case-control study included 48 newly diagnosed RA patients and 30 apparent healthy controls from two major hospitals in Ghana. Validated structured questionnaires were administered to obtain demographic data; blood samples were collected and processed for flow cytometric analysis.

Results: IFN-γ, TNF-α, IL-4, IL-6, IL-10, IL-17A, IL-6/IL-4, and IL-17/IL-10 expressions were significantly higher in RA cases compared to the healthy controls. The expression of IL-6 (0.00 (0.00-0.98) vs. 0.82 (0.34-1.10) vs. 1.56 (1.39-1.68), p < 0.0001), IL-17A (0.00 (0.00-0.02) vs. 0.19 (0.09-0.30) vs. 0.99 (0.64-1.25), p < 0.0001), and IL-17A/IL-10 (0.00 (0.00-0.39) vs. 0.15 (0.09-0.26) vs. 0.88 (0.41-1.47), p < 0.0001) increased significantly from the healthy controls through RA patients with low DAS scores to RA patients with moderate DAS scores. IL-6 (β = 0.681, r 2 = 0.527, p < 0.0001), IL-17A (β = 0.770, r 2 = 0.593, p < 0.0001), and IL-17A/IL-10 (β = 0.677, r 2 = 0.452, p < 0.0001) expressions were significantly directly associated with DAS28 scores. IL-6 (cutoff = 1.32, sensitivity = 100.0%, specificity = 100.0%, accuracy = 100.0%, and AUC = 1.000) and IL-17A (cutoff = 0.58, sensitivity = 100.0%, specificity = 100.0%, accuracy = 100.0%, and AUC = 1.000) presented with the best discriminatory power in predicting moderate DAS scores from low DAS scores.

Conclusion: Th1- and Th17-related cytokines predominate in the pathophysiology of RA, with IL-6 and IL-17 being principally and differentially expressed based on the severity of the disease. IL-6 and IL-17A could serve as useful prognostic and disease-monitoring markers in RA in the African context.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
4.40
自引率
0.00%
发文量
9
审稿时长
24 weeks
期刊最新文献
An Observational Study on the Clinical Characteristics and Prognosis of Patients With Interstitial Lung Disease Secondary to Dermatomyositis and Antisynthetase Syndrome. Exploring the Psychiatric Manifestations of Primary Sjögren’s Syndrome: A Narrative Review Association of Baseline Serum Soluble Tumour Necrosis Factor Receptor Levels with the Response of Rheumatoid Arthritis to Janus Kinase Inhibitor Therapy. Primary Immune Thrombocytopenic Purpura (ITP) and ITP Associated with Systemic Lupus Erythematosus: A Review of Clinical Characteristics and Treatment Modalities Association of Cytokine IL-17, IL-4, IL-6, and IL-12 Gene Polymorphisms in Rheumatoid Arthritis Patients in a Tertiary Care Hospital in Bangladesh.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1