一种抗3型副流感和牛呼吸道合胞病毒的鼻内活疫苗对具有母源性抗体的小牛的有效性。

IF 1.3 Q2 VETERINARY SCIENCES Veterinary Record Open Pub Date : 2020-11-06 eCollection Date: 2020-01-01 DOI:10.1136/vetreco-2020-000429
Lucy Metcalfe, Mathieu Chevalier, Marie-Pascale Tiberghien, Edmond Jolivet, Milan Huňady, Sioned Timothy, Corinne Philippe-Reversat
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引用次数: 1

摘要

试验设计:除了对欧洲犊牛的3型副流感病毒(PI3V)和牛呼吸道合胞病毒(BRSV)母源抗体(MDA)水平进行血清学调查外,还进行了两项随机对照疫苗接种试验。参与者:在两项疫苗试验中均包括有和没有MDA的10天大小牛。干预措施:鼻内注射二价改性活疫苗(PI3V/BRSV),然后在接种后大约三个月进行人工攻毒。目的:本研究旨在评估改良呼吸活疫苗Bovalto Respi鼻内疫苗(勃林格殷格翰公司)的疗效。为了评估丙二醛的干扰作用,同时使用血清阳性和血清阴性的小牛。随机化:接种前7天确定PI3V和BRSV血清学状态;没有母体抗体的小牛成为丙二醛疫苗。将感染MDA的犊牛按个体滴度排序,交替分配到MDA+接种组和MDA+对照组。盲法:由非盲法研究主任进行治疗。动物护理和兽医检查是由不知道所接受治疗的人员进行的。血清学调查使用了从五个欧洲国家(德国、西班牙、意大利、爱尔兰和英国)的商业农场获得的小牛血液样本,以确定大约两周龄的小牛中丙二醛到PI3V和BRSV的水平。结果:共36头犊牛参与了两项挑战研究,其中32头犊牛完成了挑战研究。21头小牛被纳入PI3V攻击研究,6头MDA-疫苗和7头MDA+疫苗中的6头以及5头MDA+未接种疫苗的对照小牛中的5头受到PI3V攻击。15头小牛被纳入BRSV攻击研究,5头MDA-疫苗和5头MDA+疫苗的小牛中有5头和5头MDA+疫苗的小牛中有5头受到BRSV攻击,5头MDA+疫苗未接种的对照小牛中有5头受到BRSV攻击。结果:对于这两种挑战,对照动物的临床评分和鼻脱落均显著高于接种疫苗的动物(PI3V挑战:临床评分P=0.001,鼻脱落P=0.001;BRSV攻毒:临床评分P=0.016,鼻脱落P=0.002), MDA+和MDA-接种动物在两种攻毒情况下均无显著差异(P>0.05)。在MDA血清学调查中,对来自6个国家的254个样本进行了检测。结论:在实验室条件下,攻毒研究的结果证明了该疫苗在BRSV和PI3V MDA存在下的有效性。实地评估证实,MDA阳性犊牛的MDA滴度与农场中典型的MDA滴度相符。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Efficacy of a live intranasal vaccine against parainfluenza type 3 and bovine respiratory syncytial virus in young calves with maternally derived antibodies.

Trial design: Two randomised controlled vaccination trials with artificial challenges were carried out in addition to a serological survey of levels of maternally derived antibodies (MDA) to parainfluenza type 3 virus (PI3V) and bovine respiratory syncytial virus (BRSV) in European calves.

Participants: Ten-day-old calves with and without MDA were included in the two vaccine trials.

Interventions: Intranasal administration of a bivalent modified live (PI3V/BRSV) vaccine followed by artificial challenge approximately three months post vaccination.

Objective: The study aimed to assess the efficacy of a modified live respiratory vaccine, Bovalto Respi Intranasal (Boehringer Ingelheim). In order to assess the interference of MDA, both seropositive and seronegative calves were used.

Randomisation: PI3V and BRSV serological status was determined seven days before vaccination; calves without maternal antibodies became the MDA- vaccinates. Calves with MDA were ranked according to individual titres and allocated alternately to MDA+ vaccinate and MDA+ control groups.

Blinding: Treatment was carried out by the unblinded study director. Animal care and veterinary examinations were conducted by personnel unaware of the treatments received. The serological survey used blood samples obtained from calves on commercial farms in five European countries, Germany, Spain, Italy, Ireland and the UK, to determine the levels of MDA to PI3V and BRSV in calves approximately two weeks of age.

Results: A total of 36 calves were included in the two challenge studies and 32 of these completed the challenge studies. Twenty-one calves were included in the PI3V challenge study, with six of six MDA- and six of seven MDA+ vaccinated calves and five of five MDA+ unvaccinated control calves being challenged with PI3V. Fifteen calves were included in the BRSV challenge study, with five of five MDA- and five of five MDA+ vaccinated calves and five of five MDA+ unvaccinated control calves being challenged with BRSV.

Outcome: For both challenges, clinical scores and nasal shedding were significantly higher in control animals compared with vaccinates (PI3V challenge: clinical scores P=0.001, nasal shedding P=0.001; BRSV challenge: clinical scores P=0.016, nasal shedding P=0.002) and not significantly different between MDA+ and MDA- vaccinated animals for both challenges (P>0.05). A total of 254 samples from six countries were tested in the serological survey of MDA.

Conclusion: The results of the challenge studies demonstrated the efficacy of the vaccine in the presence of BRSV and PI3V MDA under laboratory conditions. The field assessment confirmed that the MDA titres in the MDA+ calves corresponded to those typically found on farms.

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来源期刊
Veterinary Record Open
Veterinary Record Open VETERINARY SCIENCES-
CiteScore
3.00
自引率
0.00%
发文量
25
审稿时长
19 weeks
期刊介绍: Veterinary Record Open is a journal dedicated to publishing specialist veterinary research across a range of topic areas including those of a more niche and specialist nature to that considered in the weekly Vet Record. Research from all disciplines of veterinary interest will be considered. It is an Open Access journal of the British Veterinary Association.
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