Mohammad Javad Tavassolifar, Mostafa Changaei, Zahra Salehi, Fatemeh Ghasemi, Moslem Javidan, Mohammad Hossein Nicknam, Mohammad Reza Pourmand
{"title":"硫唑嘌呤可调节克罗恩病患者的氧化还原失衡。","authors":"Mohammad Javad Tavassolifar, Mostafa Changaei, Zahra Salehi, Fatemeh Ghasemi, Moslem Javidan, Mohammad Hossein Nicknam, Mohammad Reza Pourmand","doi":"10.1080/13510002.2021.1915665","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Crohn's disease (CD) is a chronic inflammatory disease without a specific cause. Inflammation in these patients can disturb the oxidants/antioxidants balance and results in oxidative stress that plays a destructive role. This study aimed to evaluate the gene expression of <i>sod1</i>, <i>sod2</i>, <i>cat</i>, <i>nrf2</i> and <i>gp91phox</i> in CD patients before and after Azathioprine (Aza) consumption.</p><p><strong>Method: </strong>Peripheral bloodmononuclear cells (PBMCs) were separated from CD patients (<i>n</i>= 15, mean age = 33.6 ± 1.8) before and after treatment with Aza and healthy controls (<i>n</i>= 15, mean age = 31.5 ± 1.2). The expression levels of <i>sod1</i>, <i>sod2</i>, <i>cat</i>, <i>nrf2</i> and <i>gp91phox</i> were measured in byusing real-time qRT-PCR technique.</p><p><strong>Result: </strong>The expression levels of <i>gp91phox</i> (<i>P-</i>value < 0.001), <i>cat</i> (<i>P-</i>value < 0.05), <i>sod1</i> (<i>P</i>-value < 0.001), <i>nrf2</i> (<i>P</i>-value < 0.001) were significantly increased compared to control group. Following treatment with Aza, the decreased expression levels of <i>gp91phox</i> (<i>P-</i>value < 0.05), <i>cat</i> (<i>P-</i>value < 0.05), <i>sod1</i>(<i>P</i>-value < 0.001) and <i>nrf2</i> (<i>P</i>-value < 0.001) were observed in CD patients.</p><p><strong>Conclusion: </strong>Overall, our results showed that prescription of Azathioprine can lead to the altered expression of redox system-related genes in patients with CD.</p>","PeriodicalId":21096,"journal":{"name":"Redox Report","volume":"26 1","pages":"80-84"},"PeriodicalIF":5.2000,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/13510002.2021.1915665","citationCount":"4","resultStr":"{\"title\":\"Redox imbalance in Crohn's disease patients is modulated by Azathioprine.\",\"authors\":\"Mohammad Javad Tavassolifar, Mostafa Changaei, Zahra Salehi, Fatemeh Ghasemi, Moslem Javidan, Mohammad Hossein Nicknam, Mohammad Reza Pourmand\",\"doi\":\"10.1080/13510002.2021.1915665\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Crohn's disease (CD) is a chronic inflammatory disease without a specific cause. Inflammation in these patients can disturb the oxidants/antioxidants balance and results in oxidative stress that plays a destructive role. This study aimed to evaluate the gene expression of <i>sod1</i>, <i>sod2</i>, <i>cat</i>, <i>nrf2</i> and <i>gp91phox</i> in CD patients before and after Azathioprine (Aza) consumption.</p><p><strong>Method: </strong>Peripheral bloodmononuclear cells (PBMCs) were separated from CD patients (<i>n</i>= 15, mean age = 33.6 ± 1.8) before and after treatment with Aza and healthy controls (<i>n</i>= 15, mean age = 31.5 ± 1.2). The expression levels of <i>sod1</i>, <i>sod2</i>, <i>cat</i>, <i>nrf2</i> and <i>gp91phox</i> were measured in byusing real-time qRT-PCR technique.</p><p><strong>Result: </strong>The expression levels of <i>gp91phox</i> (<i>P-</i>value < 0.001), <i>cat</i> (<i>P-</i>value < 0.05), <i>sod1</i> (<i>P</i>-value < 0.001), <i>nrf2</i> (<i>P</i>-value < 0.001) were significantly increased compared to control group. Following treatment with Aza, the decreased expression levels of <i>gp91phox</i> (<i>P-</i>value < 0.05), <i>cat</i> (<i>P-</i>value < 0.05), <i>sod1</i>(<i>P</i>-value < 0.001) and <i>nrf2</i> (<i>P</i>-value < 0.001) were observed in CD patients.</p><p><strong>Conclusion: </strong>Overall, our results showed that prescription of Azathioprine can lead to the altered expression of redox system-related genes in patients with CD.</p>\",\"PeriodicalId\":21096,\"journal\":{\"name\":\"Redox Report\",\"volume\":\"26 1\",\"pages\":\"80-84\"},\"PeriodicalIF\":5.2000,\"publicationDate\":\"2021-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1080/13510002.2021.1915665\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Redox Report\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/13510002.2021.1915665\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Redox Report","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/13510002.2021.1915665","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Redox imbalance in Crohn's disease patients is modulated by Azathioprine.
Background: Crohn's disease (CD) is a chronic inflammatory disease without a specific cause. Inflammation in these patients can disturb the oxidants/antioxidants balance and results in oxidative stress that plays a destructive role. This study aimed to evaluate the gene expression of sod1, sod2, cat, nrf2 and gp91phox in CD patients before and after Azathioprine (Aza) consumption.
Method: Peripheral bloodmononuclear cells (PBMCs) were separated from CD patients (n= 15, mean age = 33.6 ± 1.8) before and after treatment with Aza and healthy controls (n= 15, mean age = 31.5 ± 1.2). The expression levels of sod1, sod2, cat, nrf2 and gp91phox were measured in byusing real-time qRT-PCR technique.
Result: The expression levels of gp91phox (P-value < 0.001), cat (P-value < 0.05), sod1 (P-value < 0.001), nrf2 (P-value < 0.001) were significantly increased compared to control group. Following treatment with Aza, the decreased expression levels of gp91phox (P-value < 0.05), cat (P-value < 0.05), sod1(P-value < 0.001) and nrf2 (P-value < 0.001) were observed in CD patients.
Conclusion: Overall, our results showed that prescription of Azathioprine can lead to the altered expression of redox system-related genes in patients with CD.
期刊介绍：
Redox Report is a multidisciplinary peer-reviewed open access journal focusing on the role of free radicals, oxidative stress, activated oxygen, perioxidative and redox processes, primarily in the human environment and human pathology. Relevant papers on the animal and plant environment, biology and pathology will also be included.
While emphasis is placed upon methodological and intellectual advances underpinned by new data, the journal offers scope for review, hypotheses, critiques and other forms of discussion.
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