土壤中作物根系的x射线计算机断层扫描

Q1 Agricultural and Biological Sciences Current protocols in plant biology Pub Date : 2018-02-13 DOI:10.1002/cppb.20049

Stefan Mairhofer, Tony Pridmore, James Johnson, Darren M. Wells, Malcolm J. Bennett, Sacha J. Mooney, Craig J. Sturrock

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引用次数: 26

摘要

对土壤中植物根系结构进行量化的非破坏性方法对于帮助我们理解自然环境中影响植物根系发育的因素至关重要。随着环境变化威胁到我们为不断扩大的全球人口维持农业生产力的能力，这些方法的应用从未出现过需求的如此增长。基于x射线计算机断层扫描(CT)的表型技术允许根系的时空量化，有助于识别对非生物和生物因素的新型适应性根系结构反应。该协议报告了一个集成的工作流程，从柱准备和植物生长到根系成像和量化，使用新颖的开源软件应用程序RooTrak和RooTh。©2017 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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X-Ray Computed Tomography of Crop Plant Root Systems Grown in Soil

Non-destructive methods to quantify the root system architecture of a plant grown in soil are essential to aid our understanding of the factors that impact plant root development in natural environments. With environmental change threatening our ability to sustain agricultural productivity for an expanding global population, the application of these methods has never before seen such an increase in demand. X-ray computed tomography (CT) based phenotyping techniques permit the spatio-temporal quantification of roots, helping to identify novel adaptive root architectural responses to abiotic and biotic factors. This protocol reports an integrated workflow from column preparation and plant growth to image and quantification of the root system using novel open source software applications, RooTrak and RooTh. © 2017 by John Wiley & Sons, Inc.

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Current protocols in plant biology Agricultural and Biological Sciences-Plant Science

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. Current Protocols in Plant Biology provides reproducible step-by-step instructions for protocols relevant to plant research. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols in Plant Biology to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Plant Biology is the comprehensive source for protocols in the multidisciplinary field of plant biology, providing an extensive range of protocols from basic to cutting edge. Coverage includes: Extraction and analysis of DNA, RNA, proteins Chromosome analysis Transcriptional analysis Protein expression Metabolites Plant enzymology Epigenetics Plant genetic transformation Mutagenesis Arabidopsis, Maize, Poplar, Rice, and Soybean, and more.