韩国散装罐奶中抗β-内酰胺大肠杆菌基因特征的比较分析。

IF 2.7 2区 农林科学 Q1 VETERINARY SCIENCES Irish Veterinary Journal Pub Date : 2021-09-21 DOI:10.1186/s13620-021-00203-4
Hye-Ri Jung, Koeun Kim, Young Ju Lee
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引用次数: 3

摘要

背景:本研究分析了环境乳腺炎常见病因之一的41株β-内酰胺耐药大肠杆菌的遗传特征,从韩国三家乳制品公司运营的五家工厂的290个奶牛场的散装罐奶中分离得到。结果:对β-内酰胺抗性大肠杆菌分离株的表型和基因型特征的分析显示,即使在同一家公司内,工厂之间也存在差异。来自工厂A1和C1的分离株对第一代头孢菌素头孢噻吩表现出高耐药性(分别为76.9%和100%),而只有来自工厂B1(60.0%)、C2(66.7%)和C3(100%)的分离株表现出对四环素的耐药性。尽管所有41个β-内酰胺抗性大肠杆菌分离株都对blaOXA-1呈阳性,但blaTEM-1在来自工厂C2(100%)和C3(100%)的分离株中高度流行。在17个同时对β-内酰胺类和氨基糖苷类耐药的分离株中,最常见的多位点序列类型是ST399(13个分离株,76.5%)。此外,2个(11.8%)和12个(70.6%)分离株分别属于系统发育群B2和D,它们分别是引起肠道感染的侵袭性菌株。主要血清群是O15(70.6%),它是一种全球分布的肠外病原体。有趣的是,A1工厂的一个分离株属于O157,同时携带六个毒力基因。结论:尽管大肠杆菌分离株是从散装牛奶中分离出来的,而不是从临床乳腺炎样本中分离出来,但系统发育群B2和D以及血清群O15和O157的存在可能会对公众健康构成威胁,因为它们携带抗微生物耐药性基因和毒力因子。
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Comparative analysis of genetic characterization of β-lactam-resistant Escherichia coli from bulk tank milk in Korea.

Background: This study was conducted to analyze the genetic characteristics of 41 β-lactam-resistant Escherichia coli isolates, which are one of the common causes of environmental mastitis, isolated from the bulk tank milk of 290 dairy farms in five factories operated by three dairy companies in Korea.

Results: Analysis of the phenotypic and genotypic characteristics of β-lactam-resistant E. coli isolates revealed differences between factories even within the same company. Isolates from factory A1 and C1 showed high resistance to cephalothin (76.9 and 100%, respectively), which is a first-generation cephalosporins, whereas resistance to tetracycline was showed by only the isolates from factories B1 (60.0%), C2 (66.7%), and C3 (100%). Although all the 41 β-lactam-resistant E. coli isolates were positive for blaOXA-1, blaTEM-1 was highly prevalent in isolates from factories C2 (100%) and C3 (100%). Among 17 isolates resistant to both β-lactams and aminoglycosides, the most common multilocus sequence type was ST399 (13isolates, 76.5%). Furthermore, 2 (11.8%) and 12 (70.6%) isolates belonged to the phylogenetic groups B2 and D, respectively, which are invasive strains that cause intestinal infections, respectively. The predominant serogroup was O15 (70.6%), which is a globally distributed extraintestinal pathogen. Interestingly, one isolate from factory A1 belonged to O157 and carried six virulence genes, simultaneously.

Conclusions: Although E. coli isolates were isolated from bulk tank milk, and not the clinical mastitis samples, the presence of the phylogenetic groups B2 and D, and the serogroups O15 and O157, which harbor antimicrobial resistance genes and virulence factors, can pose a threat to public health.

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来源期刊
Irish Veterinary Journal
Irish Veterinary Journal 农林科学-兽医学
CiteScore
4.80
自引率
3.40%
发文量
1
审稿时长
>36 weeks
期刊介绍: Irish Veterinary Journal is an open access journal with a vision to make a substantial contribution to the dissemination of evidence-based knowledge that will promote optimal health and welfare of both domestic and wild species of animals. Irish Veterinary Journal has a clinical research focus with an emphasis on the effective management of health in both individual and populations of animals. Published studies will be relevant to both the international veterinary profession and veterinary scientists. Papers relating to veterinary education, veterinary ethics, veterinary public health, or relevant studies in the area of social science (participatory research) are also within the scope of Irish Veterinary Journal.
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