生物分析中基于质谱的方法和策略，用于药物相关分子的定性和定量分析

Q1 Pharmacology, Toxicology and Pharmaceutics Drug Discovery Today: Technologies Pub Date : 2021-12-01 DOI:10.1016/j.ddtec.2021.10.004

Frank Klont, Gérard Hopfgartner

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引用次数: 1

摘要

质谱法在药物相关分子的定性和定量分析中起着重要作用。本文综述了LC-MS在低分辨率和高分辨率串联质谱分析治疗性低分子量化合物、多肽和蛋白质以及寡核苷酸方面的一些最常见的应用。此外，还强调了多级质谱、差分离子迁移率和数据独立采集的好处。最后，讨论了将质谱与新型接口耦合用于高通量分析的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Mass spectrometry based approaches and strategies in bioanalysis for qualitative and quantitative analysis of pharmaceutically relevant molecules

Mass spectrometry plays an essential role in qualitative and quantitative analysis of pharmaceutically relevant molecules. The present review summarizes some the most common applications of LC-MS for the characterization of therapeutic low-molecular-weight compounds, peptides and proteins, and oligonucleotides using low-resolution and high-resolution tandem mass spectrometry. In addition, the benefit of multistage MS, differential ion mobility, and data independent acquisition is emphasized. At last, the potential of coupling MS with novel interfaces for high-throughput analysis is discussed.

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Drug Discovery Today: Technologies Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

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期刊介绍： Discovery Today: Technologies compares different technological tools and techniques used from the discovery of new drug targets through to the launch of new medicines.