Markus Hoenicka, Marius Sabau, Andreas Liebold, Hans-Stefan Hofmann, Michael Ried
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NE EC50 values were slightly lower (7.1E-8 vs. 7.5E-8, p = 0.019) after storage in HKH, with no changes after storage in the other solutions. Endothelium-dependent responses to ACh were not affected by storage. ET-1 induced contractions were attenuated after storage in HDMEM (77%, p = 0.002), HKH (75%, p = 0.020), and TiProtec (73%, p = 0.010) with no changes in normalized constrictions. ET-1 EC50 values were not affected by storage.</p><p><strong>Conclusion: </strong>Loss of contractility after storage in HDMEM may reflect the lower content of dextrose. There was no specific attenuation of adrenoceptor, ET-receptor, or ACh receptor mediated signal transduction after storage in any of the media. HKH or TiProtec are equally suitable cold storage solutions for ex vivo measurements.</p>","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Preservation of Adrenoceptor and Endothelin Receptor Mediated Vasoconstriction and of Endothelium-Dependent Relaxation after Cold Storage of Explanted Blood Vessels for ex vivo Analyses.\",\"authors\":\"Markus Hoenicka, Marius Sabau, Andreas Liebold, Hans-Stefan Hofmann, Michael Ried\",\"doi\":\"10.1159/000524922\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Adrenoceptor and endothelin (ET) receptor-mediated vasoconstriction as well as endothelium-dependent vasodilation of human saphenous veins were compared before and after 20 h of cold storage.</p><p><strong>Methods: </strong>Contractile responses to potassium chloride (KCl), norepinephrine (NE), and ET-1 as well as vasodilator responses to acetylcholine (ACh) were evaluated.</p><p><strong>Results: </strong>Storage in HEPES-supplemented Dulbecco's modified Eagle's medium (HDMEM) diminished KCl induced contractile forces to 71% (p = 0.002) and NE induced contractions to 80% (p = 0.037), in contrast to HEPES-supplemented Krebs-Henseleit solution (HKH) and TiProtec solution. 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引用次数: 0
摘要
前言:比较冷藏20 h前后人隐静脉肾上腺素受体和内皮素受体介导的血管收缩及内皮依赖性血管舒张的变化。方法:观察对氯化钾(KCl)、去甲肾上腺素(NE)、ET-1的收缩反应和对乙酰胆碱(ACh)的血管舒张反应。结果:与hepes添加的Krebs-Henseleit溶液(HKH)和TiProtec溶液相比,hpes添加的Dulbecco's modified Eagle's medium (HDMEM)中KCl诱导的收缩力减少了71% (p = 0.002), NE诱导的收缩力减少了80% (p = 0.037)。kcl归一化NE收缩不受储存影响。在HKH中保存后,NE EC50值略有降低(7.1E-8 vs. 7.5E-8, p = 0.019),在其他溶液中保存后没有变化。对乙酰胆碱的内皮依赖性反应不受储存的影响。ET-1诱导的收缩在HDMEM (77%, p = 0.002)、HKH (75%, p = 0.020)和TiProtec (73%, p = 0.010)中保存后减弱,标准化收缩无变化。ET-1 EC50值不受存储影响。结论:HDMEM储存后收缩力的丧失可能反映了葡萄糖含量的降低。在任何介质中储存后,肾上腺素受体、et受体或ACh受体介导的信号转导都没有特异性衰减。HKH或TiProtec同样适用于离体测量的冷藏解决方案。
Preservation of Adrenoceptor and Endothelin Receptor Mediated Vasoconstriction and of Endothelium-Dependent Relaxation after Cold Storage of Explanted Blood Vessels for ex vivo Analyses.
Introduction: Adrenoceptor and endothelin (ET) receptor-mediated vasoconstriction as well as endothelium-dependent vasodilation of human saphenous veins were compared before and after 20 h of cold storage.
Methods: Contractile responses to potassium chloride (KCl), norepinephrine (NE), and ET-1 as well as vasodilator responses to acetylcholine (ACh) were evaluated.
Results: Storage in HEPES-supplemented Dulbecco's modified Eagle's medium (HDMEM) diminished KCl induced contractile forces to 71% (p = 0.002) and NE induced contractions to 80% (p = 0.037), in contrast to HEPES-supplemented Krebs-Henseleit solution (HKH) and TiProtec solution. KCl-normalized NE contractions were not affected by storage. NE EC50 values were slightly lower (7.1E-8 vs. 7.5E-8, p = 0.019) after storage in HKH, with no changes after storage in the other solutions. Endothelium-dependent responses to ACh were not affected by storage. ET-1 induced contractions were attenuated after storage in HDMEM (77%, p = 0.002), HKH (75%, p = 0.020), and TiProtec (73%, p = 0.010) with no changes in normalized constrictions. ET-1 EC50 values were not affected by storage.
Conclusion: Loss of contractility after storage in HDMEM may reflect the lower content of dextrose. There was no specific attenuation of adrenoceptor, ET-receptor, or ACh receptor mediated signal transduction after storage in any of the media. HKH or TiProtec are equally suitable cold storage solutions for ex vivo measurements.
期刊介绍:
The ''Journal of Vascular Research'' publishes original articles and reviews of scientific excellence in vascular and microvascular biology, physiology and pathophysiology. The scope of the journal covers a broad spectrum of vascular and lymphatic research, including vascular structure, vascular function, haemodynamics, mechanics, cell signalling, intercellular communication, growth and differentiation. JVR''s ''Vascular Update'' series regularly presents state-of-the-art reviews on hot topics in vascular biology. Manuscript processing times are, consistent with stringent review, kept as short as possible due to electronic submission. All articles are published online first, ensuring rapid publication. The ''Journal of Vascular Research'' is the official journal of the European Society for Microcirculation. A biennial prize is awarded to the authors of the best paper published in the journal over the previous two years, thus encouraging young scientists working in the exciting field of vascular biology to publish their findings.