[四氧嘧啶型糖尿病大鼠肝脏琥珀酸脱氢酶的分子生化研究]

Q3 Biochemistry, Genetics and Molecular Biology Biomeditsinskaya khimiya Pub Date : 2022-08-01 DOI:10.18097/PBMC20226804272
A T Eprintsev, D N Fedorin, M Yu Bakarev
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引用次数: 1

摘要

实验性四氧嘧啶糖尿病引起大鼠肝脏琥珀酸脱氢酶(SDH)活性增加,但其同工酶组成未发生变化。所观察到的SDH催化活性的增加显然与编码SDH催化二聚体的基因转录的增强有关。在正常和实验条件下,对大鼠SDH催化二聚体编码基因启动子的甲基状态分析未发现其表达水平有任何依赖性。亚硫酸酯测序结果表明,SDH基因表达调控在四氧嘧啶型糖尿病的发生发展中具有被动的表观遗传机制。在糖尿病中负责糖异生的转录因子CREB可能在控制sdha和sdhb基因的转录活性中起重要作用。
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[Molecular and biochemical studies of succinate dehydrogenase in rat liver under conditions of alloxan diabetes].

Experimental alloxan diabetes in rats causes an increase in the activity of liver succinate dehydrogenase (SDH) without changes in its isozyme composition. The observed increase in the catalytic activity of SDH clearly correlates with the intensification of transcription of the genes encoding catalytic dimer of SDH. Analysis of the methyl status of the promoters of the genes, encoding the catalytic dimer of SDH in rats under normal and experimental conditions did not reveal any dependence on the level of their expression. The obtained results of bisulfite sequencing indicate a passive role of the epigenetic mechanism of regulation of SDH gene expression in the development of alloxan diabetes. The transcription factor CREB, responsible for of gluconeogenesis in diabetes, may play an important role in the control of the transcriptional activity of the sdha and sdhb genes.

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来源期刊
Biomeditsinskaya khimiya
Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍: The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).
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