{"title":"sonnei志贺氏菌外膜蛋白在开发基于抗体的诊断志贺氏菌病的免疫测定中的作用。","authors":"N N F N Mahadi, C Y Leow, K K Banga Singh","doi":"10.47665/tb.39.2.015","DOIUrl":null,"url":null,"abstract":"<p><p>Conventional culture method and biochemical tests remain as the 'gold standard' method for the identification of S. sonnei which are time-consuming. We have discovered previously the potential of three OMPs of S. sonnei (33.3 kDa, 43.8 kDa and 100.3 kDa) as biomarkers in the diagnostic test for shigellosis. Here, we evaluated the performance of the outer membrane proteins of S. sonnei for the development of an antibody-based immunoassay for the detection of S. sonnei infections. All threetarget proteins were specifically recognized when probed with S. sonnei sera. In addition, another two potential proteins of molecular weight 29.0 kDa and 88.2 kDa in size were also exclusively recognized by the IgA when probed with S. sonnei sera. The optimized ELISA demonstrated higher sensitivity and specificity which exceeded 86.0%. In conclusion, the identified target proteins showed great potential as diagnostic biomarkers for the detection of S. sonnei infections in patients.</p>","PeriodicalId":23476,"journal":{"name":"Tropical biomedicine","volume":"39 2","pages":"252-256"},"PeriodicalIF":0.8000,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Usefulness of the outer membrane proteins of Shigella sonnei in developing an antibody-based immunoassay for the diagnosis of shigellosis.\",\"authors\":\"N N F N Mahadi, C Y Leow, K K Banga Singh\",\"doi\":\"10.47665/tb.39.2.015\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Conventional culture method and biochemical tests remain as the 'gold standard' method for the identification of S. sonnei which are time-consuming. We have discovered previously the potential of three OMPs of S. sonnei (33.3 kDa, 43.8 kDa and 100.3 kDa) as biomarkers in the diagnostic test for shigellosis. Here, we evaluated the performance of the outer membrane proteins of S. sonnei for the development of an antibody-based immunoassay for the detection of S. sonnei infections. All threetarget proteins were specifically recognized when probed with S. sonnei sera. In addition, another two potential proteins of molecular weight 29.0 kDa and 88.2 kDa in size were also exclusively recognized by the IgA when probed with S. sonnei sera. The optimized ELISA demonstrated higher sensitivity and specificity which exceeded 86.0%. In conclusion, the identified target proteins showed great potential as diagnostic biomarkers for the detection of S. sonnei infections in patients.</p>\",\"PeriodicalId\":23476,\"journal\":{\"name\":\"Tropical biomedicine\",\"volume\":\"39 2\",\"pages\":\"252-256\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2022-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tropical biomedicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.47665/tb.39.2.015\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical biomedicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.47665/tb.39.2.015","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Usefulness of the outer membrane proteins of Shigella sonnei in developing an antibody-based immunoassay for the diagnosis of shigellosis.
Conventional culture method and biochemical tests remain as the 'gold standard' method for the identification of S. sonnei which are time-consuming. We have discovered previously the potential of three OMPs of S. sonnei (33.3 kDa, 43.8 kDa and 100.3 kDa) as biomarkers in the diagnostic test for shigellosis. Here, we evaluated the performance of the outer membrane proteins of S. sonnei for the development of an antibody-based immunoassay for the detection of S. sonnei infections. All threetarget proteins were specifically recognized when probed with S. sonnei sera. In addition, another two potential proteins of molecular weight 29.0 kDa and 88.2 kDa in size were also exclusively recognized by the IgA when probed with S. sonnei sera. The optimized ELISA demonstrated higher sensitivity and specificity which exceeded 86.0%. In conclusion, the identified target proteins showed great potential as diagnostic biomarkers for the detection of S. sonnei infections in patients.
期刊介绍：
The Society publishes the Journal – Tropical Biomedicine, 4 issues yearly. It was first started in 1984. The journal is now abstracted / indexed by Medline, ISI Thompson, CAB International, Zoological Abstracts, SCOPUS. It is available free on the MSPTM website. Members may submit articles on Parasitology, Tropical Medicine and other related subjects for publication in the journal subject to scrutiny by referees. There is a charge of US$200 per manuscript. However, charges will be waived if the first author or corresponding author are members of MSPTM of at least three (3) years'' standing.
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