{"title":"emx2下游增强子CNE2.04在斑马鱼中的组织特异性相关区域和基序","authors":"Xudong Chen, Qi Zhang, Jia Lin, Yinglan Zhang, Yawen Zhang, Yiting Gui, Ruizhi Zhang, Ting Liu, Qiang Li","doi":"10.1016/j.gep.2022.119269","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Expression level of <em>EMX2</em><span> plays an important role in the development of nervous system and cancers. CNE2.04, a conserved enhancer downstream of </span><em>emx2</em><span>, drives fluorescent protein expression in the similar pattern of </span><em>emx2</em>.</p></div><div><h3>Methods</h3><p>CNE2.04 truncated or motif-mutated transgenic reporter plasmids were constructed and injected into the zebrafish fertilized egg with Tol2 mRNA at the unicellular stage of zebrafish eggs. The green fluorescence expression patterns were observed at 24, 48, and 72 hpf, and the fluorescence rates of different tissues were counted at 48 hpf.</p></div><div><h3>Results</h3><p>Compared to CNE2.04, CNE2.04-R400 had comparable enhancer activity, while the tissue specificity of CNE2.04-L400 was obviously changed. Motif CCCCTC mutation obviously changed the enhancer activity, while motif CCGCTC mutations also changed it.</p></div><div><h3>Conclusion</h3><p>Due to their correlation with tissue specificity, CNE2.04-R400 is associated with the tissue-specificity of CNE2.04, and motif CCCCTC plays an important role in the enhancer activity of CNE2.04.</p></div>","PeriodicalId":55598,"journal":{"name":"Gene Expression Patterns","volume":"45 ","pages":"Article 119269"},"PeriodicalIF":1.0000,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The tissue-specificity associated region and motif of an emx2 downstream enhancer CNE2.04 in zebrafish\",\"authors\":\"Xudong Chen, Qi Zhang, Jia Lin, Yinglan Zhang, Yawen Zhang, Yiting Gui, Ruizhi Zhang, Ting Liu, Qiang Li\",\"doi\":\"10.1016/j.gep.2022.119269\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Expression level of <em>EMX2</em><span> plays an important role in the development of nervous system and cancers. CNE2.04, a conserved enhancer downstream of </span><em>emx2</em><span>, drives fluorescent protein expression in the similar pattern of </span><em>emx2</em>.</p></div><div><h3>Methods</h3><p>CNE2.04 truncated or motif-mutated transgenic reporter plasmids were constructed and injected into the zebrafish fertilized egg with Tol2 mRNA at the unicellular stage of zebrafish eggs. The green fluorescence expression patterns were observed at 24, 48, and 72 hpf, and the fluorescence rates of different tissues were counted at 48 hpf.</p></div><div><h3>Results</h3><p>Compared to CNE2.04, CNE2.04-R400 had comparable enhancer activity, while the tissue specificity of CNE2.04-L400 was obviously changed. Motif CCCCTC mutation obviously changed the enhancer activity, while motif CCGCTC mutations also changed it.</p></div><div><h3>Conclusion</h3><p>Due to their correlation with tissue specificity, CNE2.04-R400 is associated with the tissue-specificity of CNE2.04, and motif CCCCTC plays an important role in the enhancer activity of CNE2.04.</p></div>\",\"PeriodicalId\":55598,\"journal\":{\"name\":\"Gene Expression Patterns\",\"volume\":\"45 \",\"pages\":\"Article 119269\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2022-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gene Expression Patterns\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1567133X22000394\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"DEVELOPMENTAL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene Expression Patterns","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1567133X22000394","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}
The tissue-specificity associated region and motif of an emx2 downstream enhancer CNE2.04 in zebrafish
Background
Expression level of EMX2 plays an important role in the development of nervous system and cancers. CNE2.04, a conserved enhancer downstream of emx2, drives fluorescent protein expression in the similar pattern of emx2.
Methods
CNE2.04 truncated or motif-mutated transgenic reporter plasmids were constructed and injected into the zebrafish fertilized egg with Tol2 mRNA at the unicellular stage of zebrafish eggs. The green fluorescence expression patterns were observed at 24, 48, and 72 hpf, and the fluorescence rates of different tissues were counted at 48 hpf.
Results
Compared to CNE2.04, CNE2.04-R400 had comparable enhancer activity, while the tissue specificity of CNE2.04-L400 was obviously changed. Motif CCCCTC mutation obviously changed the enhancer activity, while motif CCGCTC mutations also changed it.
Conclusion
Due to their correlation with tissue specificity, CNE2.04-R400 is associated with the tissue-specificity of CNE2.04, and motif CCCCTC plays an important role in the enhancer activity of CNE2.04.
期刊介绍：
Gene Expression Patterns is devoted to the rapid publication of high quality studies of gene expression in development. Studies using cell culture are also suitable if clearly relevant to development, e.g., analysis of key regulatory genes or of gene sets in the maintenance or differentiation of stem cells. Key areas of interest include:
-In-situ studies such as expression patterns of important or interesting genes at all levels, including transcription and protein expression
-Temporal studies of large gene sets during development
-Transgenic studies to study cell lineage in tissue formation
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