连接蛋白43在鸡发育过程中定位于砂囊平滑肌细胞。

IF 1.8 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Journal of Poultry Science Pub Date : 2022-10-25 DOI:10.2141/jpsa.0220003
Kosuke Tokunaga, Shota Akimoto, Machiko Aiba, Mutsuki Nakagomi, Takahiro Suzuki, Ryuichi Tatsumi, Mako Nakamura
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引用次数: 1

摘要

平滑肌细胞广泛分布于鸡的消化器官中。它们以单细胞的形式存在,但相互结合以同步发挥作用。本研究研究了鸡胚期(E) 10、E15和E18时鸡胗中间隙连接蛋白连接蛋白43 (Cx43)的表达。砂囊有大量的平滑肌细胞,因为它们的肌肉层很厚,可以很容易地分析细胞。确认了平滑肌标记物的形态学观察和表达模式。接下来,我们观察了标记在E10, E15和E18的砂囊组织中的定位。最后,研究了Cx43在原代培养的E15砂囊平滑肌细胞中的表达规律。分析揭示了Cx43和calponin 1在平滑肌层的表达和定位,3D分析揭示了Cx43在E10 ~ E15的定位模式的动态变化。培养的平滑肌细胞证实Cx43在细胞膜和胞浆中表达。综上所述,Cx43在鸡胗E10、E15和E18位点均有表达,但在发育过程中定位不同。在E10表达广泛,在E15和E18表达受限。平滑肌细胞原代培养表明,Cx43存在于细胞膜和细胞质中。这表明Cx43在E10被主动翻译到细胞质中,形成六聚体,并穿梭于细胞膜中作为间隙连接。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Connexin 43 is Localized in Gizzard Smooth Muscle Cells during Chicken Development.

Smooth muscle cells are widely distributed in the digestive organs of chickens. They exist as single cells, but adhere to each other to function synchronously. In this study, the expression of the gap junction protein connexin 43 (Cx43) in chicken gizzards was investigated at embryonic days (E) 10, E15, and E18. Gizzards have an abundance of smooth muscle cells because of their thick muscle layers, which enable easy analysis of the cells. Morphological observations and expression patterns of smooth muscle markers were confirmed. Next, we observed where the markers were localized in the gizzard tissue at E10, E15, and E18. Finally, the expression pattern of Cx43 in primary cultured smooth muscle cells from E15 gizzards was investigated. The analysis revealed the expression and localization of Cx43 and calponin 1 in the smooth muscle layers, and 3D analysis revealed dynamic changes in the localization pattern of Cx43 from E10 to E15. Cultured smooth muscle cells confirmed that Cx43 was expressed in the cell membrane and cytosol. In conclusion, Cx43 expression was identified in chicken gizzards at E10, E15, and E18, which was localized differently during development. The expression was broad at E10, and became restricted at E15 and E18. Primary culture of smooth muscle cells showed that Cx43 was present in the cell membrane and cytosol. This suggests that Cx43 is actively translated into the cytosol at E10, forming a hexamer, and shuttling the cell membrane to function as a gap junction.

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来源期刊
Journal of Poultry Science
Journal of Poultry Science AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
2.80
自引率
13.30%
发文量
26
审稿时长
12 months
期刊介绍: The Journal of Poultry Science will publish original reports and reviews which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: breeding and genetics, nutrition and feeds, physiology, reproduction, immunology, behavior, environmental science, management and housing welfare, processing and products, and health in poultry. Submission of original articles to the Journal is open to all poultry researchers. The review articles are invited papers written by international outstanding researchers. Articles will be published in English, American style.
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