URINARY PROTEIN AND CARBOHYDRATE. I. FRACTIONATION OF NONDIALYZABLE COMPONENTS OF HUMAN URINE.

Summary 1. A simple preliminary fractionation method for the nondialyzable urinary carbohydrate containing solids is reported. Sodium chloride is added to urine to 0.58 M to precipitate CPC-I (Tamm and Horsfall mucoid). After dialysis and concentration, CPC-II is separated from AMP and glycopeptide with addition of (NH4)2SO4 to 100% saturation. 2. The present method enables (a) simultaneous quantitative evaluation of 3 carbohydrate-containing fractions in urine, (b) determination of the glycopeptide and AMP content of the AMP fraction, and (c) further fractionation of these into individual components. 3. The mean and standard error of the daily excretion of each component for all samples was: CPC-I—50 ±6mg; CPC-II—193 ± 23 mg; AMP— 13 ± 2 mg; and glycopeptide—15 ± 2 mg. With increasing age, the excretion of CPC-II is increased. The excretion of AMP is highest between 10–20 yrs of age. It decreases thereafter.