Mayumi Matsubara, Aya Masaoka, Tamon Tanaka, Hiroaki Terato, Yoshihiko Ohyama, Hiroshi Ide
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Identification and characterization of mammalian 5-formyluracil-DNA glycosylase.
5-Formyluracil is a major oxidative thymine lesion with mutagenic and cytotoxic properties. In this study, we have partially purified and characterized a mammalian 5-formyluracil-DNA glycosylase (FDG) from rat liver. FDG was a monofunctional DNA glycosylase and removed 5-formyluracil, uracil, 5-hydroxyuracil, 5-hydroxylmethyluracil in single-stranded and double-stranded DNA. Several lines of evidence indicate that FDG is a rat SMUG1 homologue. Human SMUG1 also exhibited similar enzymatic properties.
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