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引用次数: 0
摘要
作为我们最近利用反义寡核苷酸-金属络合物(es)偶联物进行RNA切割研究的一部分，我们用三吡啶x Cu(II)络合物制备了自互补的2'- o -甲基寡核苷酸，该络合物连接在5'端或3'端的糖部分。与未修饰的2'- o -甲基双相物相比，所形成的双相物的热稳定性表明，5'-和3'-配合物都大大提高了双相的稳定性。
Oligonucleotide duplex stabilization by end-linked terpyridine x Cu(II) complexes.
As a part of our recent studies of RNA cleavage using antisense oligonucleotide-metal complex(es) conjugates, we prepared self-complementary 2'-O-methyloligonucleotides with a terpyridine x Cu(II) complex, which was attached to the sugar portion of the 5'-end or 3'-end. The thermal stabilities of the formed duplexes, as compared with those of the unmodified 2'-O-methyl duplexes, revealed that both the 5'- and 3'-complexes greatly enhanced the duplex stability.
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