{"title":"4'-硫核糖核苷酸(4'-thioRNA)物理生理性质的研究。","authors":"Shuichi Hoshika, Naonori Inoue, Noriaki Minakawa, Akira Matsuda","doi":"10.1093/nass/3.1.209","DOIUrl":null,"url":null,"abstract":"<p><p>An efficient and practical synthesis of 4'-thioribonucleosides was accomplished via the Pummerer reaction. The resulting 4'-thioribonucleosides were converted into the corresponding phosphoramidite units, and 4'-thioribonucleic acids (4'-thioRNAs) of 15 mer were synthesized on a DNA synthesizer using a controlled pore glass (CPG) with 4'-thiouridine unit. The thermal stability of 4'-thioRNA:RNA duplex was higher than that of RNA:RNA duplex. Moreover, the thermal stability of 4'-thioRNA:4'-thioRNA duplex was the highest and stabilized 30 degrees C or more compared with RNA:RNA duplex (>99 degrees C vs 66 degrees C). Structural analysis by CD spectra indicates that 4'-thioRNA:4'-thioRNA duplex and 4'-thioRNA:RNA duplex adapt A-form conformation as well as RNA:RNA duplex.</p>","PeriodicalId":86149,"journal":{"name":"Nucleic acids research. Supplement (2001)","volume":" 3","pages":"209-10"},"PeriodicalIF":0.0000,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/3.1.209","citationCount":"3","resultStr":"{\"title\":\"Investigation of physical and physiological properties of 4'-thioribonucleotide (4'-thioRNA).\",\"authors\":\"Shuichi Hoshika, Naonori Inoue, Noriaki Minakawa, Akira Matsuda\",\"doi\":\"10.1093/nass/3.1.209\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>An efficient and practical synthesis of 4'-thioribonucleosides was accomplished via the Pummerer reaction. The resulting 4'-thioribonucleosides were converted into the corresponding phosphoramidite units, and 4'-thioribonucleic acids (4'-thioRNAs) of 15 mer were synthesized on a DNA synthesizer using a controlled pore glass (CPG) with 4'-thiouridine unit. The thermal stability of 4'-thioRNA:RNA duplex was higher than that of RNA:RNA duplex. Moreover, the thermal stability of 4'-thioRNA:4'-thioRNA duplex was the highest and stabilized 30 degrees C or more compared with RNA:RNA duplex (>99 degrees C vs 66 degrees C). Structural analysis by CD spectra indicates that 4'-thioRNA:4'-thioRNA duplex and 4'-thioRNA:RNA duplex adapt A-form conformation as well as RNA:RNA duplex.</p>\",\"PeriodicalId\":86149,\"journal\":{\"name\":\"Nucleic acids research. Supplement (2001)\",\"volume\":\" 3\",\"pages\":\"209-10\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2003-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1093/nass/3.1.209\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nucleic acids research. Supplement (2001)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1093/nass/3.1.209\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nucleic acids research. Supplement (2001)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/nass/3.1.209","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Investigation of physical and physiological properties of 4'-thioribonucleotide (4'-thioRNA).
An efficient and practical synthesis of 4'-thioribonucleosides was accomplished via the Pummerer reaction. The resulting 4'-thioribonucleosides were converted into the corresponding phosphoramidite units, and 4'-thioribonucleic acids (4'-thioRNAs) of 15 mer were synthesized on a DNA synthesizer using a controlled pore glass (CPG) with 4'-thiouridine unit. The thermal stability of 4'-thioRNA:RNA duplex was higher than that of RNA:RNA duplex. Moreover, the thermal stability of 4'-thioRNA:4'-thioRNA duplex was the highest and stabilized 30 degrees C or more compared with RNA:RNA duplex (>99 degrees C vs 66 degrees C). Structural analysis by CD spectra indicates that 4'-thioRNA:4'-thioRNA duplex and 4'-thioRNA:RNA duplex adapt A-form conformation as well as RNA:RNA duplex.
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