用MagiProbe检测单碱基错配的特异性

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI:10.1093/nass/3.1.165

Akio Yamane, Nozomi Nagata

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引用次数: 0

摘要

magprobe已经被证明是一种均匀型的探针，在杂交时发出荧光，并具有单碱基错配的特异性。为了获得更全面的错配识别特性信息，对MagiProbe在不同序列背景下的8个单碱基错配进行了错配识别测试。结果表明，碱基错配的识别主要受错配碱基组合的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Specificity of one-base mismatch detection with MagiProbe.

MagiProbe have been demonstrated to be a homogeneous type of probe that emits fluorescence upon hybridization and with specificity for one-base mismatch. To obtain more comprehensive information for the properties of mismatch recognition, examination of mismatch recognition of MagiProbe for the eight single-base mismatches in variety of sequence contexts was performed. The results indicate that the recognition of base mismatches is influenced predominantly by combination of mispaired bases.

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Nucleic acids research. Supplement (2001)

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