Ya-Ru Chen, Hong-Jia Yang, Jin-Myong Cha, Xin-Xin Zhang, Dong Fan
{"title":"KaSPI在粘虫中的表达模式及抗真菌功能研究。","authors":"Ya-Ru Chen, Hong-Jia Yang, Jin-Myong Cha, Xin-Xin Zhang, Dong Fan","doi":"10.1017/S000748532300041X","DOIUrl":null,"url":null,"abstract":"<p><p>Kazal-type serine protease inhibitors (KaSPI) play important roles in insect growth, development, digestion, metabolism and immune defence. In this study, based on the transcriptome of <i>Mythimna separata</i>, the cDNA sequence of <i>MsKaSPI</i> with Kazal domain was uploaded to GenBank (MN931651). Spatial and temporal expression analysis showed that <i>MsKaSPI</i> was expressed at different developmental stages and different tissues, and it was induced by 20-hydroxyecdysone in third-instar larvae of <i>M. separata</i>. After 24 h infection by <i>Beauveria bassiana</i>, the expression level of <i>MsKaSPI</i> and the corresponding MsKaSPI content were significantly up-regulated, being 6.42-fold and 1.91-fold to the control group, respectively, while the activities of serine protease, trypsin and chymotrypsin were inhibited. After RNA interference interfered with <i>MsKaSPI</i> for 6 h, the expression decreased by 73.44%, the corresponding content of MsKaSPI protein decreased by 55.66% after 12 h, and the activities of serine protease and trypsin were significantly enhanced. Meanwhile, both the larval and pupal stages of <i>M. separata</i> were prolonged, the weights were reduced and the number of eggs per female decreased by 181. <i>Beauveria bassiana</i> infection also increased the mortality of <i>MsKaSPI</i>-silenced <i>M. separata</i> by 18.96%. These prove <i>MsKaSPI</i> can not only result in slow growth and low fecundity of <i>M. separata</i> by regulating the activity of related protease, but also participate in the resistance to pathogenic fungi by regulating the serine protease inhibitor content and the activities of related serine protease.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"756-766"},"PeriodicalIF":1.6000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Expression patterns and antifungal function study of KaSPI in <i>Mythimna separata</i>.\",\"authors\":\"Ya-Ru Chen, Hong-Jia Yang, Jin-Myong Cha, Xin-Xin Zhang, Dong Fan\",\"doi\":\"10.1017/S000748532300041X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Kazal-type serine protease inhibitors (KaSPI) play important roles in insect growth, development, digestion, metabolism and immune defence. In this study, based on the transcriptome of <i>Mythimna separata</i>, the cDNA sequence of <i>MsKaSPI</i> with Kazal domain was uploaded to GenBank (MN931651). Spatial and temporal expression analysis showed that <i>MsKaSPI</i> was expressed at different developmental stages and different tissues, and it was induced by 20-hydroxyecdysone in third-instar larvae of <i>M. separata</i>. After 24 h infection by <i>Beauveria bassiana</i>, the expression level of <i>MsKaSPI</i> and the corresponding MsKaSPI content were significantly up-regulated, being 6.42-fold and 1.91-fold to the control group, respectively, while the activities of serine protease, trypsin and chymotrypsin were inhibited. After RNA interference interfered with <i>MsKaSPI</i> for 6 h, the expression decreased by 73.44%, the corresponding content of MsKaSPI protein decreased by 55.66% after 12 h, and the activities of serine protease and trypsin were significantly enhanced. Meanwhile, both the larval and pupal stages of <i>M. separata</i> were prolonged, the weights were reduced and the number of eggs per female decreased by 181. <i>Beauveria bassiana</i> infection also increased the mortality of <i>MsKaSPI</i>-silenced <i>M. separata</i> by 18.96%. These prove <i>MsKaSPI</i> can not only result in slow growth and low fecundity of <i>M. separata</i> by regulating the activity of related protease, but also participate in the resistance to pathogenic fungi by regulating the serine protease inhibitor content and the activities of related serine protease.</p>\",\"PeriodicalId\":9370,\"journal\":{\"name\":\"Bulletin of Entomological Research\",\"volume\":\" \",\"pages\":\"756-766\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2023-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bulletin of Entomological Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1017/S000748532300041X\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/9/21 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Entomological Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1017/S000748532300041X","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/9/21 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Expression patterns and antifungal function study of KaSPI in Mythimna separata.
Kazal-type serine protease inhibitors (KaSPI) play important roles in insect growth, development, digestion, metabolism and immune defence. In this study, based on the transcriptome of Mythimna separata, the cDNA sequence of MsKaSPI with Kazal domain was uploaded to GenBank (MN931651). Spatial and temporal expression analysis showed that MsKaSPI was expressed at different developmental stages and different tissues, and it was induced by 20-hydroxyecdysone in third-instar larvae of M. separata. After 24 h infection by Beauveria bassiana, the expression level of MsKaSPI and the corresponding MsKaSPI content were significantly up-regulated, being 6.42-fold and 1.91-fold to the control group, respectively, while the activities of serine protease, trypsin and chymotrypsin were inhibited. After RNA interference interfered with MsKaSPI for 6 h, the expression decreased by 73.44%, the corresponding content of MsKaSPI protein decreased by 55.66% after 12 h, and the activities of serine protease and trypsin were significantly enhanced. Meanwhile, both the larval and pupal stages of M. separata were prolonged, the weights were reduced and the number of eggs per female decreased by 181. Beauveria bassiana infection also increased the mortality of MsKaSPI-silenced M. separata by 18.96%. These prove MsKaSPI can not only result in slow growth and low fecundity of M. separata by regulating the activity of related protease, but also participate in the resistance to pathogenic fungi by regulating the serine protease inhibitor content and the activities of related serine protease.
期刊介绍:
Established in 1910, the internationally recognised Bulletin of Entomological Research aims to further global knowledge of entomology through the generalisation of research findings rather than providing more entomological exceptions. The Bulletin publishes high quality and original research papers, ''critiques'' and review articles concerning insects or other arthropods of economic importance in agriculture, forestry, stored products, biological control, medicine, animal health and natural resource management. The scope of papers addresses the biology, ecology, behaviour, physiology and systematics of individuals and populations, with a particular emphasis upon the major current and emerging pests of agriculture, horticulture and forestry, and vectors of human and animal diseases. This includes the interactions between species (plants, hosts for parasites, natural enemies and whole communities), novel methodological developments, including molecular biology, in an applied context. The Bulletin does not publish the results of pesticide testing or traditional taxonomic revisions.