单核细胞来源的树突状细胞的功能和自噬受到乙型肝炎病毒感染的影响。

IF 2.9 4区 医学 Q3 IMMUNOLOGY BMC Immunology Pub Date : 2023-09-26 DOI:10.1186/s12865-023-00571-2
Hua Xu, Juan Kang, Shan Zhong, Min Chen, Peng Hu, Hong Ren, Zhi Zhou, Yu Lei
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摘要

背景:树突状细胞和树突状细胞的自噬状态在乙型肝炎病毒(HBV)感染的免疫反应中的作用仍然存在争议。在本研究中,我们仔细检查了HBV感染中树突状细胞的表型、功能和自噬途径。方法:分别用脂多糖、HepG2.2.15细胞上清液和HepG2细胞上清液刺激健康献血者和慢性HBV感染者的单核细胞来源的树突状细胞。流式细胞仪检测树突状细胞表型,酶联免疫吸附法检测细胞因子分泌。通过蛋白质印迹和免疫荧光分析检测自噬相关蛋白。结果:主要组织相容性复合体II分子和共刺激分子(包括分化抗原簇80,当与HepG2.2.15细胞的上清液培养时,来自慢性HBV感染患者的单核细胞衍生的树突状细胞中的分化抗原簇86显著高于来自健康供体的分化抗原。当HBV刺激时,慢性HBV感染患者的单核细胞衍生的树突状细胞分泌的细胞因子,包括肿瘤坏死因子-α、白细胞介素-10和白细胞介质-12的量也显著高于健康供体。有趣的是,与健康供体相比,慢性HBV感染患者树突状细胞中自噬相关蛋白(包括自噬相关蛋白质5和相关蛋白质1轻链)的表达水平显著升高呈递抗原并表达共刺激分子。树突状细胞活化的增加可能与HBV感染中树突状细胞自噬的增强有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Function and autophagy of monocyte-derived dendritic cells is affected by hepatitis B virus infection.

Background: The role of dendritic cells and the autophagy state of dendritic cells in the immune response of hepatitis B virus (HBV) infection was still controversial. In this study, we carefully examined the phenotype, function and autophagy pathway of dendritic cells in HBV infection.

Methods: Monocyte-derived dendritic cells from healthy blood donors and patients with chronic HBV infection were stimulated by lipopolysaccharide, supernatant of HepG2.2.15 cells or supernatant of HepG2 cells respectively. Phenotype of dendritic cells was examined by flow cytometry and cytokines secretion was detected by enzyme-linked immunosorbent assay. Autophagy related proteins were detected by western blot and immunofluorescence analysis.

Results: Our results showed that the expression of both major histocompatibility complex II molecules and co-stimulated molecules including cluster of differentiation antigen 80, cluster of differentiation antigen 86 in the monocyte-derived dendritic cells from patients with chronic HBV infection was significantly higher than that from healthy donors when cultured with supernatant of HepG2.2.15 cells. The amount of cytokines, including tumour necrosis factor-α, interleukin-10 and interleukin-12, secreted by monocyte-derived dendritic cells from patients with chronic HBV infection was also significantly higher than that from healthy donors when stimulate by HBV. Interestingly, the expression level of autophagy-related proteins including autophagy-related protein5 and associated protein 1 light chain in dendritic cells from patients with chronic HBV infection was significantly increased when compared with that from healthy donors when re-exposed to HBV.

Conclusions: Our results indicated that dendritic cells from patients with chronic HBV infection could intensively present antigen and express co-stimulatory molecules. The increased activation of dendritic cells might be related to the enhanced autophagy of dendritic cells in HBV infection.

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来源期刊
BMC Immunology
BMC Immunology 医学-免疫学
CiteScore
5.50
自引率
0.00%
发文量
54
审稿时长
1 months
期刊介绍: BMC Immunology is an open access journal publishing original peer-reviewed research articles in molecular, cellular, tissue-level, organismal, functional, and developmental aspects of the immune system as well as clinical studies and animal models of human diseases.
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