SA生物合成中的一个正反馈调节环SA-AtNAP-SAG202/SARD1-IC1-SA参与叶片衰老,但不参与防御反应。

IF 10.6 Q1 HORTICULTURE Molecular Horticulture Pub Date : 2022-06-17 DOI:10.1186/s43897-022-00036-x
Yaxin Wang, Bin Liu, Youzhen Hu, Su-Sheng Gan
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引用次数: 0

摘要

水杨酸(SA)是一种重要的植物激素,调节防御反应和叶片衰老。必须了解调节SA生物合成基因的上游因素。SAG202/SARD1是防御反应中等容酸合成酶1(ICS1)诱导和SA生物合成的关键调节因子。SA生物合成在叶片衰老过程中的调控机制尚不清楚。在这里,我们发现AtNAP,一种衰老特异性NAC家族转录因子,直接调节名为SAG202的衰老相关基因,如在酵母一个杂交种和植物分析中所揭示的。诱导的AtNAP和SAG202的过度表达导致叶片中SA的高水平和早衰。sag202和ics1的个体敲除突变体具有显著降低的SA水平,并表现出显著延迟的叶片衰老表型。此外,SA正反馈调节AtNAP和SAG202。我们的研究发现了一个独特的正反馈调节回路,SA-AtNAP-SAG02-ICS1-SA,其作用是控制与叶片衰老相关的SA生物合成,而不是防御反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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A positive feedback regulatory loop, SA-AtNAP-SAG202/SARD1-ICS1-SA, in SA biosynthesis involved in leaf senescence but not defense response.

Salicylic acid (SA) is an important plant hormone that regulates defense responses and leaf senescence. It is imperative to understand upstream factors that regulate genes of SA biosynthesis. SAG202/SARD1 is a key regulator for isochorismate synthase 1 (ICS1) induction and SA biosynthesis in defense responses. The regulatory mechanism of SA biosynthesis during leaf senescence is not well understood. Here we show that AtNAP, a senescence-specific NAC family transcription factor, directly regulates a senescence-associated gene named SAG202 as revealed in yeast one-hybrid and in planta assays. Inducible overexpreesion of AtNAP and SAG202 lead to high levels of SA and precocious senescence in leaves. Individual knockout mutants of sag202 and ics1 have markedly reduced SA levels and display a significantly delayed leaf senescence phenotype. Furthermore, SA positively feedback regulates AtNAP and SAG202. Our research has uncovered a unique positive feedback regulatory loop, SA-AtNAP-SAG202-ICS1-SA, that operates to control SA biosynthesis associated with leaf senescence but not defense response.

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来源期刊
Molecular Horticulture
Molecular Horticulture horticultural research-
CiteScore
8.00
自引率
0.00%
发文量
24
审稿时长
12 weeks
期刊介绍: Aims Molecular Horticulture aims to publish research and review articles that significantly advance our knowledge in understanding how the horticultural crops or their parts operate mechanistically. Articles should have profound impacts not only in terms of high citation number or the like, but more importantly on the direction of the horticultural research field. Scope Molecular Horticulture publishes original Research Articles, Letters, and Reviews on novel discoveries on the following, but not limited to, aspects of horticultural plants (including medicinal plants): ▪ Developmental and evolutionary biology ▪ Physiology, biochemistry and cell biology ▪ Plant-microbe and plant-environment interactions ▪ Genetics and epigenetics ▪ Molecular breeding and biotechnology ▪ Secondary metabolism and synthetic biology ▪ Multi-omics dealing with data sets of genome, transcriptome, proteome, metabolome, epigenome and/or microbiome. The journal also welcomes research articles using model plants that reveal mechanisms and/or principles readily applicable to horticultural plants, translational research articles involving application of basic knowledge (including those of model plants) to the horticultural crops, novel Methods and Resources of broad interest. In addition, the journal publishes Editorial, News and View, and Commentary and Perspective on current, significant events and topics in global horticultural fields with international interests.
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