阪崎Cronobacter sakazakii和金黄色葡萄球菌的共培养:探讨混合生物膜形成的影响和阪崎克罗诺杆菌生物膜形成基因的调控。

Danliangmin Song, Ai Jia, Xuehe Qi, Kai Dong, Shiyu Liu, Chaoxin Man, Xinyan Yang, Yujun Jiang
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引用次数: 1

摘要

细菌生物膜是由包裹细菌的细菌分泌的代谢产物组成的保护性基质。通过形成生物膜,细菌可以显著提高其对环境的耐受性。在与食品相关的加工环境中,不同类型的微生物通常存在于生物膜中。婴儿配方奶粉(PIF)加工环境中的主要污染菌株阪崎克罗诺杆菌和金黄色葡萄球菌在生物膜生产后继续污染PIF加工环境。本研究选择生物膜形成能力较弱的阪崎克罗诺杆菌作为试验生物之一。模拟了阪崎Cronobacter sakazakii和金黄色葡萄球菌在生产设备表面共存的情况,分析了相互作用。共培养组的生物膜形成显著高于其他组。金黄色葡萄球菌对阪崎克罗诺杆菌生物膜形成基因影响的深入研究。结果表明,两种细菌可以共存于金属器件表面,形成更紧凑的混合生物膜结构。在共培养条件下,金黄色葡萄球菌增加了阪崎克罗诺杆菌中bcsA和fliD的表达,而降低了bcsC的表达。金黄色葡萄球菌(Autoinducer 2)产生的信号分子在0-500 ng/mL(0.099-0.177)浓度下显著促进阪崎克罗诺杆菌的生物膜形成,并上调bcsA、filD和flhD基因的表达。
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Co-culture of Cronobacter sakazakii and Staphylococcus aureus: Explore the influence of mixed biofilm formation and regulation of Cronobacter sakazakii biofilm formation genes.

Bacterial biofilm is a protective matrix composed of metabolites secreted by bacteria that envelop bacteria. By forming a biofilm, bacteria can considerably improve their environmental tolerance. In food-related processing environment, different types of microorganisms are often present in biofilms. The main contaminating strain in the powdered infant formula (PIF) processing environment, Cronobacter sakazakii and Staphylococcus aureus continues to pollute the PIF processing environment after biofilm production. This study selected Cronobacter sakazakii with a weak biofilm-forming ability as one of the test organisms. The coexistence of Cronobacter sakazakii and Staphylococcus aureus on the surface of production equipment was simulated to analyze the interaction. Biofilm formation in the co-culture group was significantly higher than the others. In-depth study of the effect of Staphylococcus aureus on the biofilm formation genes of Cronobacter sakazakii. Results show two bacteria can coexist on the surface of a metal device, forming a more compact hybrid biofilm structure. Under co-culture conditions, S. aureus increased bcsA and fliD expression in Cronobacter sakazakii, whereas decreased bcsC expression. Signaling molecules produced by Staphylococcus aureus (Autoinducer 2) significantly promoted the biofilm formation of Cronobacter sakazakii at the concentration of 0-500 ng/mL (0.099-0.177) and up-regulated the expression of bcsA, filD and flhD genes.

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