Hussam A. Al Saoud, Michał Szumski, Myroslav Sprynskyy, Szymon Bocian, Bogusław Buszewski
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The aim of this work was to use the biosilica obtained from diatoms (microalgae) cultivated under laboratory conditions as a new stationary phase to fill the chromatographic column and test it in high-performance liquid chromatography. Biosilica is an inorganic polymer consisting of orthosilicate units formed by organisms such as diatoms or siliceous sponges. The results showed that the prepared columns were characterized by relatively high efficiency, comparable to a commercially available material HALO HILIC of dp = 2.7 μm. The retention of polar compounds under a high acetonitrile content was worse than that on HALO HILIC, but it was proportionally lower when the surface area of both materials was compared. As model test solutes, nucleosides and nucleobases have undergone testing. They were examined separately for retention, and attempts to separate test mixtures were successful.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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