恒河猴子宫内膜异位症的治疗方案。

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES Journal of Medical Primatology Pub Date : 2023-10-17 DOI:10.1111/jmp.12681

Ov D. Slayden, Fangzhou Luo, Lauren D. Martin, D.V.M

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引用次数: 0

摘要

背景：子宫内膜异位症是指子宫腔外存在子宫内膜样组织。通过子宫颈收集和腹腔镜接种月经子宫内膜，在狒狒身上建立了子宫内膜异位症的实验模型。猕猴是药物开发的首选模型，但猕猴子宫颈的复杂解剖结构使狒狒的方法不切实际。在这项工作中，我们试图验证一种在猕猴中制造子宫内膜异位症的手术方法。方法：经腹腔镜宫腔穿刺收集月经期子宫内膜，转移至腹膜腔。我们在三次月经期间重复了这个程序。子宫内膜异位组织在腹腔镜检查中被鉴定、收集并通过免疫组织化学进行表征。结果：假手术治疗的动物（n = 3）未能发展为子宫内膜异位症。我们在13/14只接受治疗的动物中发现了红色、粉末烧伤和白色病变；红色病变的基质对卵巢类固醇受体呈阳性染色。结论：该手术技术可以可靠地在猕猴体内制造激素反应性子宫内膜异位症，用于治疗研究。

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A protocol for creating endometriosis in rhesus macaques (Macaca mulatta)

Background

Endometriosis is the presence of endometrium-like tissue outside the uterine cavity. An experimental model of endometriosis has been created in the baboon by the transcervical collection and laparoscopic inoculation of menstrual endometrium. Macaques are the preferred model for pharmaceutical development, but the complex anatomy of the macaque cervix makes the baboon method impractical. In this work, we sought to validate a surgical approach for creating endometriosis in macaques.

Methods

Menstrual endometrium was collected via laparoscopic intrauterine puncture and transferred to the peritoneal cavity. We repeated this procedure during three menstruations. Endometriotic tissue was identified during laparoscopy, collected, and characterized by immunohistochemistry.

Results

Sham surgery-treated animals (n = 3) failed to develop endometriosis. We identified red, powder burnt, and white lesions in 13/14 of the treated animals; the stroma of the red lesions stained positive for ovarian steroid receptors.

Conclusion

This surgical technique can reliably create hormone-responsive endometriosis in macaques for therapeutic studies.

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来源期刊

Journal of Medical Primatology 生物-动物学

CiteScore

1.40

自引率

42.90%

发文量

审稿时长

6 months

期刊介绍： The Journal of Medical Primatology publishes research on non-human primates as models to study, prevent, and/or treat human diseases; subjects include veterinary medicine; morphology, physiology, reproductive biology, central nervous system, and cardiovascular diseases; husbandry, handling, experimental methodology, and management of non-human primate colonies and laboratories; non-human primate wildlife management; and behaviour and sociology as related to medical conditions and captive non-human primate needs. Published material includes: Original Manuscripts - research results; Case Reports - scientific documentation of a single clinical study; Short Papers - case histories, methodologies, and techniques of particular interest; Letters to the Editor - opinions, controversies and sporadic scientific observations; Perspectives – opinion piece about existing research on a particular topic; Minireviews – a concise review of existing literature; Book Reviews by invitation; Special Issues containing selected papers from specialized meetings; and Editorials and memoriams authored by the Editor-in-Chief.