Linke Zhong , Tingting Fu , Chengdong Wang , Xufeng Qi , Wai-Yee Chan , Dongqing Cai , Hui Zhao
{"title":"热带爪蟾早期胚胎发育过程中过氧氧化还原素基因家族的发育表达。","authors":"Linke Zhong , Tingting Fu , Chengdong Wang , Xufeng Qi , Wai-Yee Chan , Dongqing Cai , Hui Zhao","doi":"10.1016/j.gep.2023.119345","DOIUrl":null,"url":null,"abstract":"<div><p><em>Peroxidase</em> genes (<em>Prdx</em>) encode a family of antioxidant proteins, which can protect cells from oxidative damage by reducing various cellular peroxides. This study investigated the spatiotemporal expression patterns of gene members in this family during the early development of <em>Xenopus tropicalis</em>. Real-time quantitative PCR showed that all members of this gene family have a distinct temporal expression pattern during the early development of <em>X. tropicalis</em> embryos. Additionally, whole mount in situ hybridization revealed that individual <em>prdx</em> genes display differential expression patterns, with overlapping expression in lymphatic vessels, pronephros, proximal tubule, and branchial arches. This study provides a basis for further study of the function of the <em>prdx</em> gene family.</p></div>","PeriodicalId":55598,"journal":{"name":"Gene Expression Patterns","volume":"50 ","pages":"Article 119345"},"PeriodicalIF":1.0000,"publicationDate":"2023-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Developmental expression of peroxiredoxin gene family in early embryonic development of Xenopus tropicalis\",\"authors\":\"Linke Zhong , Tingting Fu , Chengdong Wang , Xufeng Qi , Wai-Yee Chan , Dongqing Cai , Hui Zhao\",\"doi\":\"10.1016/j.gep.2023.119345\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Peroxidase</em> genes (<em>Prdx</em>) encode a family of antioxidant proteins, which can protect cells from oxidative damage by reducing various cellular peroxides. This study investigated the spatiotemporal expression patterns of gene members in this family during the early development of <em>Xenopus tropicalis</em>. Real-time quantitative PCR showed that all members of this gene family have a distinct temporal expression pattern during the early development of <em>X. tropicalis</em> embryos. Additionally, whole mount in situ hybridization revealed that individual <em>prdx</em> genes display differential expression patterns, with overlapping expression in lymphatic vessels, pronephros, proximal tubule, and branchial arches. This study provides a basis for further study of the function of the <em>prdx</em> gene family.</p></div>\",\"PeriodicalId\":55598,\"journal\":{\"name\":\"Gene Expression Patterns\",\"volume\":\"50 \",\"pages\":\"Article 119345\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2023-10-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gene Expression Patterns\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1567133X2300042X\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"DEVELOPMENTAL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene Expression Patterns","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1567133X2300042X","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}
Developmental expression of peroxiredoxin gene family in early embryonic development of Xenopus tropicalis
Peroxidase genes (Prdx) encode a family of antioxidant proteins, which can protect cells from oxidative damage by reducing various cellular peroxides. This study investigated the spatiotemporal expression patterns of gene members in this family during the early development of Xenopus tropicalis. Real-time quantitative PCR showed that all members of this gene family have a distinct temporal expression pattern during the early development of X. tropicalis embryos. Additionally, whole mount in situ hybridization revealed that individual prdx genes display differential expression patterns, with overlapping expression in lymphatic vessels, pronephros, proximal tubule, and branchial arches. This study provides a basis for further study of the function of the prdx gene family.
期刊介绍:
Gene Expression Patterns is devoted to the rapid publication of high quality studies of gene expression in development. Studies using cell culture are also suitable if clearly relevant to development, e.g., analysis of key regulatory genes or of gene sets in the maintenance or differentiation of stem cells. Key areas of interest include:
-In-situ studies such as expression patterns of important or interesting genes at all levels, including transcription and protein expression
-Temporal studies of large gene sets during development
-Transgenic studies to study cell lineage in tissue formation