In vitro evaluation of acaricidal activity of aqueous ozone against Dermanyssus gallina

The aim of this study was to investigate the in vitro acaricidal effect of aqueous ozone against Dermanyssus gallinae (Acari: Dermanyssidae) under laboratory conditions. For this purpose, aqueous ozone at concentrations of 1, 2, 3, 4, 5 and 10 ppm were prepared, and five replicate experiments were carried out using 400 µl of each concentration sprayed on every treatment batch of mites (plus a distilled water control). The mortality rate of mites in treatment and control groups were assessed 24 h post exposure. The mortality rate obtained by concentrations of 4, 5, and 10 ppm were significantly different from the control group (p < 0.05). The highest mortality rate (63.99%) was observed at concentration of 10 ppm. In this study, aqueous ozone showed a dose-dependent acaricidal potency against D. gallinae.