Otimização das condições de cultivo do fungo filamentoso Fusarium sp. EA 1.3.7 para a produção de xilanases

Xylanases are enzymes widely used in industrial processes, such as paper bleaching, the bakery industry, juice and beer clarification, malting, and the production of biofuels, due to the benefits and advantages of the biological catalyst. In this context, we aimed to bioprospecting filamentous fungi, select a potential xylanase-producing fungus, optimize the enzymatic production, and perform the biochemical characterization of the enzyme. Eleven filamentous fungi with distinct morphology were isolated from three samples of organic materials collected in the soil of the city of Diamantina, Minas Gerais, Brazil, and Fusarium sp. EA 1.3.7 was selected as a potential xylanase-producer. The best growth conditions for the production of xylanase were standardized in a submerged CP medium, for six days, at 30oC, using peptone as a nitrogen source, and wheat bran as a carbon source; the xylanases produced showed greater activities at 52oC and pH 5.3.