Microbial Diversity and Abundance in Pulmonary Tissue of Patients with Early-Stage Lung Cancer

Background: The composition of lung tissue microorganisms in patients with different tissue types of lung cancer has not yet been determined. Previous studies have shown changes in the composition of pulmonary microbial flora in patients with lung cancer. Objectives: This study aimed to investigate the differences and correlations in the microbial flora of pulmonary tissue among different histological types of lung cancer. Methods: Samples of tumor and normal lung tissue from 29 patients with early-stage lung cancer were collected. The samples were sequenced using Illumina HiSeq high throughput sequencing technology for 16S rDNA in the V4 region of the bacteria. Also, their microbiological characteristics were detected, and bioinformatics analysis was performed. Results: The results of microbial abundance analysis in the lung tissue of patients with lung cancer showed that the bacterial colony composition of the two tissues was similar, with Proteobacteria, Thickwalled Bacteria, Anomalococcus, Bacteroides, and Actinobacteria predominating. Analysis of microbial diversity found no difference in α diversity and β diversity between normal lung tissue and tumor tissue. When analyzing patients with adenocarcinoma, the abundance of Micrococcales and Blastomonas was significantly higher in tumor tissue than in normal lung tissue. Conclusions: The composition of microbial flora in different parts of lung tissue of early-stage lung cancer patients is consistent, but the dominant flora varies among different histological types of lung cancer.