经宫颈标本中滋养细胞的检测和分离方法-历史概述

Q4 Biochemistry, Genetics and Molecular Biology Medical Journal of Cell Biology Pub Date : 2021-12-01 DOI:10.2478/acb-2021-0024
Rafał Sibiak, E. Wender-Ożegowska
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引用次数: 0

摘要

滋养细胞可以从宫颈上皮细胞中检测和分离,通过各种经宫颈样本收集技术,如粘液抽吸、宫颈灌洗或标准宫颈刷拭,在妊娠早期,甚至从妊娠5周开始。分离的胎儿细胞可用于胎儿性别的早期预测,最常见的非整倍体的产前诊断,以及任何其他遗传异常。然而,与目前使用的检测母体循环中游离胎儿DNA的方法或妊娠后期可用的其他侵入性产前诊断方案相比,滋养层细胞的收集效果有限。过去几年,经宫颈细胞样本主要采集于计划终止妊娠前的妇女。妊娠期妇女早期滋养层细胞的分离为进一步研究揭示许多妊娠相关并发症的病理生理学开辟了新的视角。
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Methods of detection and isolation of trophoblast cells from trans-cervical specimens – a historical overview
Abstract Trophoblast cells can be detected and isolated from the cervical epithelial cells obtained via various techniques of trans-cervical samples collection such as a mucus aspiration, endocervical lavage, or standard cervical brushing in the early first trimester, even from the 5 weeks’ gestation. Isolated fetal cells can be used in the early prediction of fetal sex, prenatal diagnostics of the most common aneuploidies, and any other genetic abnormalities. Nevertheless, the collection of trophoblastic cells has limited efficacy compared to currently used methods of detection of free fetal DNA in maternal circulation or other protocols of invasive prenatal diagnostics available at later stages of pregnancy. In the past years, trans-cervical cell samples were collected mainly in women before planned pregnancy termination. The early trophoblastic cells isolation from women in ongoing pregnancies opens new perspectives for further studies focused on the elucidation of pathophysiology of numerous pregnancy-related complications.
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Medical Journal of Cell Biology
Medical Journal of Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology
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