Research progress on autophagy detection

Autophagy is one of hotspots in the biomedical field. It has been shown that autophagy is related to the pathogenesis of various diseases. The autophagic flux is a process from the formation of the barrier membrane to the autolysis of the lysosome to complete the hydrolysis function, which is a complete, multistep and dynamic autophagy process. Therefore, autophagic flux can be used as a measure of autophagy level, and qualitative and quantitative detection of autophagic flux can be achieved by combining experimental methods of dynamic and static. It can reflect the level of autophagy more accurately and objectively. This article intends to give a brief introduction to the detection of autophagy, and especially describes various detection methods of autophagic flux, in order to provide technical support for the majority of related researchers. Key words: Autophagy; Clinical laboratory techniques; Microscopy, electron; Microscopy, fluorescence; Biological markers; Plasmids; Autophagy flux