Risha Fillah Fithria, Budi Aryono, Muhammad Zainuddin
{"title":"不同启蒙强度的钝顶螺旋藻抑制剂对蛋白质含量、色素率和抗氧化活性的影响","authors":"Risha Fillah Fithria, Budi Aryono, Muhammad Zainuddin","doi":"10.14710/jmr.v11i4.36432","DOIUrl":null,"url":null,"abstract":"Intensitas cahaya merupakan faktor penting dalam fotosintesis. Tujuan penelitian adalah melakukan optimasi intensitas pencahayaan kultur Spirulina platensis untuk mendapatkan pertumbuhan, protein, biopigmen dan aktivitas antioksidan tertinggi. Hasil penelitian menunjukkan bahwa perlakuan perbedaan intensitas pencahayaan berpengaruh terhadap kultur Spirulina platensis. Perlakuan terbaik adalah intensitas 9000 lux yaitu pertumbuhan mutlak sebesar 1,948 d x 104 sel/ml, laju pertumbuhan 0,171 hari-1, jumlah generasi 2,215, waktu generasi 4,067 hari, yield 5,764 gr/gr, produktifitas 21,347 gr/L.hari, protein 12,61 %. Ekstrak Spirulina platensis mengandung klorofil a sebesar 9,187 mg/L, klorofil b 6,679 mg/L, total klorofil 17,167 mg/L, karotenoid 3,685 mg/L, fikosianin 0,112 mg/L, alofikosianin 0,014 mg/L, fikoeritrin 0,285 mg/L. Eksrak Spirulina platensis memiliki aktivitas antioksidan dengan nilai IC50 sebesar 111,5 ppm. Aktivitas perendaman radikal DPPH dari ekstrak dikarenakan adanya senyawa antioksidan dari klorofil, karotenoid dan fikobiliprotein. Light intensity is an important factor in photosynthesis. The aim of the research was to optimize the lighting intensity of Spirulina platensis culture to obtain the highest growth, protein, biopigment and antioxidant activity. The treatment of different lighting intensity affects the culture of Spirulina platensis. The best treatment was the intensity of 9000 lux, namely absolute growth of 1.948 d x 104 cell/ml, growth rate of 0.171 day-1, number of generations of 2.215, generation time of 4.067 days, yield of 5.764 gr/gr, productivity 21.347 gr/L.day, protein 12,61%. Spirulina platensis extract contains chlorophyll a 9.187 mg/L, chlorophyll b 6.679 mg/L, total chlorophyll 17,167 mg/L, carotenoids 3.685 mg/L, phycocyanin 0.112 mg/L, allophycocyanin 0.014 mg/L, phycoerythrin 0.285 mg/L. Spirulina platensis extract has antioxidant activity with an IC50 value of 111.5 ppm. The DPPH radical scavenging activity of the extract was due to the presence of antioxidant compounds from chlorophyll, carotenoids and phycobiliproteins.","PeriodicalId":50153,"journal":{"name":"Journal of Marine Research","volume":" ","pages":""},"PeriodicalIF":0.5000,"publicationDate":"2022-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pengaruh Intensitas Pencahayaan Yang Berbeda Pada Kultur Spirulina platensis Terhadap Kandungan Protein, Kadar Pigmen Dan Aktivitas Antioksidan\",\"authors\":\"Risha Fillah Fithria, Budi Aryono, Muhammad Zainuddin\",\"doi\":\"10.14710/jmr.v11i4.36432\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Intensitas cahaya merupakan faktor penting dalam fotosintesis. Tujuan penelitian adalah melakukan optimasi intensitas pencahayaan kultur Spirulina platensis untuk mendapatkan pertumbuhan, protein, biopigmen dan aktivitas antioksidan tertinggi. Hasil penelitian menunjukkan bahwa perlakuan perbedaan intensitas pencahayaan berpengaruh terhadap kultur Spirulina platensis. Perlakuan terbaik adalah intensitas 9000 lux yaitu pertumbuhan mutlak sebesar 1,948 d x 104 sel/ml, laju pertumbuhan 0,171 hari-1, jumlah generasi 2,215, waktu generasi 4,067 hari, yield 5,764 gr/gr, produktifitas 21,347 gr/L.hari, protein 12,61 %. Ekstrak Spirulina platensis mengandung klorofil a sebesar 9,187 mg/L, klorofil b 6,679 mg/L, total klorofil 17,167 mg/L, karotenoid 3,685 mg/L, fikosianin 0,112 mg/L, alofikosianin 0,014 mg/L, fikoeritrin 0,285 mg/L. Eksrak Spirulina platensis memiliki aktivitas antioksidan dengan nilai IC50 sebesar 111,5 ppm. Aktivitas perendaman radikal DPPH dari ekstrak dikarenakan adanya senyawa antioksidan dari klorofil, karotenoid dan fikobiliprotein. Light intensity is an important factor in photosynthesis. The aim of the research was to optimize the lighting intensity of Spirulina platensis culture to obtain the highest growth, protein, biopigment and antioxidant activity. The treatment of different lighting intensity affects the culture of Spirulina platensis. The best treatment was the intensity of 9000 lux, namely absolute growth of 1.948 d x 104 cell/ml, growth rate of 0.171 day-1, number of generations of 2.215, generation time of 4.067 days, yield of 5.764 gr/gr, productivity 21.347 gr/L.day, protein 12,61%. Spirulina platensis extract contains chlorophyll a 9.187 mg/L, chlorophyll b 6.679 mg/L, total chlorophyll 17,167 mg/L, carotenoids 3.685 mg/L, phycocyanin 0.112 mg/L, allophycocyanin 0.014 mg/L, phycoerythrin 0.285 mg/L. Spirulina platensis extract has antioxidant activity with an IC50 value of 111.5 ppm. 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Pengaruh Intensitas Pencahayaan Yang Berbeda Pada Kultur Spirulina platensis Terhadap Kandungan Protein, Kadar Pigmen Dan Aktivitas Antioksidan
Intensitas cahaya merupakan faktor penting dalam fotosintesis. Tujuan penelitian adalah melakukan optimasi intensitas pencahayaan kultur Spirulina platensis untuk mendapatkan pertumbuhan, protein, biopigmen dan aktivitas antioksidan tertinggi. Hasil penelitian menunjukkan bahwa perlakuan perbedaan intensitas pencahayaan berpengaruh terhadap kultur Spirulina platensis. Perlakuan terbaik adalah intensitas 9000 lux yaitu pertumbuhan mutlak sebesar 1,948 d x 104 sel/ml, laju pertumbuhan 0,171 hari-1, jumlah generasi 2,215, waktu generasi 4,067 hari, yield 5,764 gr/gr, produktifitas 21,347 gr/L.hari, protein 12,61 %. Ekstrak Spirulina platensis mengandung klorofil a sebesar 9,187 mg/L, klorofil b 6,679 mg/L, total klorofil 17,167 mg/L, karotenoid 3,685 mg/L, fikosianin 0,112 mg/L, alofikosianin 0,014 mg/L, fikoeritrin 0,285 mg/L. Eksrak Spirulina platensis memiliki aktivitas antioksidan dengan nilai IC50 sebesar 111,5 ppm. Aktivitas perendaman radikal DPPH dari ekstrak dikarenakan adanya senyawa antioksidan dari klorofil, karotenoid dan fikobiliprotein. Light intensity is an important factor in photosynthesis. The aim of the research was to optimize the lighting intensity of Spirulina platensis culture to obtain the highest growth, protein, biopigment and antioxidant activity. The treatment of different lighting intensity affects the culture of Spirulina platensis. The best treatment was the intensity of 9000 lux, namely absolute growth of 1.948 d x 104 cell/ml, growth rate of 0.171 day-1, number of generations of 2.215, generation time of 4.067 days, yield of 5.764 gr/gr, productivity 21.347 gr/L.day, protein 12,61%. Spirulina platensis extract contains chlorophyll a 9.187 mg/L, chlorophyll b 6.679 mg/L, total chlorophyll 17,167 mg/L, carotenoids 3.685 mg/L, phycocyanin 0.112 mg/L, allophycocyanin 0.014 mg/L, phycoerythrin 0.285 mg/L. Spirulina platensis extract has antioxidant activity with an IC50 value of 111.5 ppm. The DPPH radical scavenging activity of the extract was due to the presence of antioxidant compounds from chlorophyll, carotenoids and phycobiliproteins.
期刊介绍:
The Journal of Marine Research publishes peer-reviewed research articles covering a broad array of topics in physical, biological and chemical oceanography. Articles that deal with processes, as well as those that report significant observations, are welcome. In the area of biology, studies involving coupling between ecological and physical processes are preferred over those that report systematics. Authors benefit from thorough reviews of their manuscripts, where an attempt is made to maximize clarity. The time between submission and publication is kept to a minimum; there is no page charge.