不同启蒙强度的钝顶螺旋藻抑制剂对蛋白质含量、色素率和抗氧化活性的影响

IF 0.5 4区 地球科学 Q3 Earth and Planetary Sciences Journal of Marine Research Pub Date : 2022-11-08 DOI:10.14710/jmr.v11i4.36432
Risha Fillah Fithria, Budi Aryono, Muhammad Zainuddin
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引用次数: 0

摘要

光照强度是光合作用的一个重要因素。该研究的目的是优化培养光照的强度,以实现螺旋藻的生长、蛋白质、生物色素和最高的抗氧化活性。研究表明,不同光照强度的处理会影响螺旋藻的培养。最佳实践是9000勒克斯强度,即1948天×104个细胞/ml的绝对生长,0171天-1的生长速率,世代数2215,世代时间4067天,产量5764克/克,生产力21347克/升,蛋白质12,61%。螺旋藻提取物含有9.187 mg/L叶绿素、6.679 mg/L叶绿素、17.167 mg/L总叶绿素、3.685 mg/L类胡萝卜素、0.112 mg/L氟菌素、0.014 mg/L阿洛氟菌素和0.285 mg/L氟菌灵。螺旋藻提取物具有抗氧化活性,IC50值为111.5ppm。由于叶绿素、类胡萝卜素和脂蛋白中的抗氧化化合物的存在,提取物具有自由基DPPH印刷活性。光照强度是光合作用的一个重要因素。本研究的目的是优化培养螺旋藻的光照强度,以获得最高的生长、蛋白质、生物色素和抗氧化活性。不同光照强度的处理对钝顶螺旋藻的培养有影响。最佳处理强度为9000lux,即绝对生长1.948d×104个细胞/ml,生长速率0.171天-1,世代数2.215,世代时间4.067天,产量5.764gr/g,生产力21.347gr/L天,蛋白质12.61%。螺旋藻提取物含有叶绿素a 9187mg/L、叶绿素b 6679mg/L、总叶绿素17167mg/L、类胡萝卜素3685mg/L、藻蓝蛋白0112mg/L、别藻蓝蛋白0014mg/L、藻红蛋白0285mg/L。螺旋藻提取物具有抗氧化活性,IC50值为111.5ppm。提取物的DPPH自由基清除活性是由于叶绿素、类胡萝卜素和藻胆蛋白中存在抗氧化化合物。
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Pengaruh Intensitas Pencahayaan Yang Berbeda Pada Kultur Spirulina platensis Terhadap Kandungan Protein, Kadar Pigmen Dan Aktivitas Antioksidan
Intensitas cahaya merupakan faktor penting dalam fotosintesis. Tujuan penelitian adalah melakukan optimasi intensitas pencahayaan kultur Spirulina platensis untuk mendapatkan pertumbuhan, protein, biopigmen dan aktivitas antioksidan tertinggi. Hasil penelitian menunjukkan bahwa perlakuan perbedaan intensitas pencahayaan berpengaruh terhadap kultur Spirulina platensis. Perlakuan terbaik adalah intensitas 9000 lux yaitu pertumbuhan mutlak sebesar 1,948 d x 104 sel/ml, laju pertumbuhan 0,171 hari-1, jumlah generasi 2,215, waktu generasi 4,067 hari, yield 5,764 gr/gr, produktifitas 21,347 gr/L.hari, protein 12,61 %. Ekstrak Spirulina platensis mengandung klorofil a sebesar 9,187 mg/L, klorofil b 6,679 mg/L, total klorofil 17,167 mg/L, karotenoid 3,685 mg/L, fikosianin 0,112 mg/L, alofikosianin 0,014 mg/L, fikoeritrin 0,285 mg/L. Eksrak Spirulina platensis memiliki aktivitas antioksidan dengan nilai IC50 sebesar 111,5 ppm. Aktivitas perendaman radikal DPPH dari ekstrak dikarenakan adanya senyawa antioksidan dari klorofil, karotenoid dan fikobiliprotein.  Light intensity is an important factor in photosynthesis. The aim of the research was to optimize the lighting intensity of Spirulina platensis culture to obtain the highest growth, protein, biopigment and antioxidant activity. The treatment of different lighting intensity affects the culture of Spirulina platensis. The best treatment was the intensity of 9000 lux, namely absolute growth of 1.948 d x 104 cell/ml, growth rate of 0.171 day-1, number of generations of 2.215, generation time of 4.067 days, yield of 5.764 gr/gr, productivity 21.347 gr/L.day, protein 12,61%. Spirulina platensis extract contains chlorophyll a 9.187 mg/L, chlorophyll b 6.679 mg/L, total chlorophyll 17,167 mg/L, carotenoids 3.685 mg/L, phycocyanin 0.112 mg/L, allophycocyanin 0.014 mg/L, phycoerythrin 0.285 mg/L. Spirulina platensis extract has antioxidant activity with an IC50 value of 111.5 ppm. The DPPH radical scavenging activity of the extract was due to the presence of antioxidant compounds from chlorophyll, carotenoids and phycobiliproteins.
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Journal of Marine Research
Journal of Marine Research 地学-海洋学
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3 months
期刊介绍: The Journal of Marine Research publishes peer-reviewed research articles covering a broad array of topics in physical, biological and chemical oceanography. Articles that deal with processes, as well as those that report significant observations, are welcome. In the area of biology, studies involving coupling between ecological and physical processes are preferred over those that report systematics. Authors benefit from thorough reviews of their manuscripts, where an attempt is made to maximize clarity. The time between submission and publication is kept to a minimum; there is no page charge.
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