{"title":"在Gilts的整个发情周期中评估下丘脑Kisspeptin系统","authors":"J. Clapper, Eric Jolitz, W. Dhillo","doi":"10.4236/ojas.2021.114040","DOIUrl":null,"url":null,"abstract":"Kisspeptin \nhas been demonstrated to affect reproductive cyclicity and the attainment of \npuberty in multiple species, presumably through its actions on gonadotropin \nreleasing hormone and luteinizing hormone. Kisspeptin administration causes increased \nplasma concentrations of LH in pigs, sheep, and rats. The objective of this \nexperiment was to evaluate changes in the hypothalamic kisspeptin system \nthroughout the estrous cycle in gilts. Estrus was synchronized in forty \ncrossbred gilts (191 d, 121 kg) and estrus detection was performed by exposing \ngilts to a mature boar. The first day gilts stood immobile was denoted d 1 of \nthe estrous cycle. Blood samples were collected via jugular venipuncture on d \n1, 4, 7, 9, 14, 16, and 19 of the estrous cycle. Ten animals were slaughtered \non d 1, 9, 14, and 21 of the estrous cycle when medial basal hypothalami, \nanterior pituitary glands, and blood were collected. Relative expression of \nhypothalamic kisspeptin (KISS1), kisspeptin receptor (KISS1R), estrogen receptors-a, \nanterior pituitary gland GnRH receptor, β-actin, and GAPDH was determined \nusing real-time reverse transcriptase PCR. Fold changes in relative expression \nwere determined using the Relative Expression Software Tool. Relative \nexpression of KISS1 was increased (P = 0.006) 3.2 fold on d 1 versus d \n21 and 2.3 fold (P = 0.003) on d 9 versus d 21 of the estrous cycle, but \nwas not different (P > 0.05) among the remaining days of the estrous \ncycle. Relative expression of estrogen receptor-b was decreased (P = \n0.05) 0.8 fold on d 9 versus d 21 and (P = 0.005) 0.7 fold on d 14 \nversus d 21, but was not different (P > 0.05) among the remaining \ndays. Relative expression of anterior pituitary gland GnRH receptor was \nincreased (P","PeriodicalId":62784,"journal":{"name":"动物科学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of the Hypothalamic Kisspeptin System throughout the Estrous Cycle in Gilts\",\"authors\":\"J. Clapper, Eric Jolitz, W. Dhillo\",\"doi\":\"10.4236/ojas.2021.114040\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Kisspeptin \\nhas been demonstrated to affect reproductive cyclicity and the attainment of \\npuberty in multiple species, presumably through its actions on gonadotropin \\nreleasing hormone and luteinizing hormone. Kisspeptin administration causes increased \\nplasma concentrations of LH in pigs, sheep, and rats. The objective of this \\nexperiment was to evaluate changes in the hypothalamic kisspeptin system \\nthroughout the estrous cycle in gilts. Estrus was synchronized in forty \\ncrossbred gilts (191 d, 121 kg) and estrus detection was performed by exposing \\ngilts to a mature boar. The first day gilts stood immobile was denoted d 1 of \\nthe estrous cycle. Blood samples were collected via jugular venipuncture on d \\n1, 4, 7, 9, 14, 16, and 19 of the estrous cycle. Ten animals were slaughtered \\non d 1, 9, 14, and 21 of the estrous cycle when medial basal hypothalami, \\nanterior pituitary glands, and blood were collected. Relative expression of \\nhypothalamic kisspeptin (KISS1), kisspeptin receptor (KISS1R), estrogen receptors-a, \\nanterior pituitary gland GnRH receptor, β-actin, and GAPDH was determined \\nusing real-time reverse transcriptase PCR. Fold changes in relative expression \\nwere determined using the Relative Expression Software Tool. Relative \\nexpression of KISS1 was increased (P = 0.006) 3.2 fold on d 1 versus d \\n21 and 2.3 fold (P = 0.003) on d 9 versus d 21 of the estrous cycle, but \\nwas not different (P > 0.05) among the remaining days of the estrous \\ncycle. Relative expression of estrogen receptor-b was decreased (P = \\n0.05) 0.8 fold on d 9 versus d 21 and (P = 0.005) 0.7 fold on d 14 \\nversus d 21, but was not different (P > 0.05) among the remaining \\ndays. Relative expression of anterior pituitary gland GnRH receptor was \\nincreased (P\",\"PeriodicalId\":62784,\"journal\":{\"name\":\"动物科学期刊(英文)\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-08-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"动物科学期刊(英文)\",\"FirstCategoryId\":\"1091\",\"ListUrlMain\":\"https://doi.org/10.4236/ojas.2021.114040\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"动物科学期刊(英文)","FirstCategoryId":"1091","ListUrlMain":"https://doi.org/10.4236/ojas.2021.114040","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Evaluation of the Hypothalamic Kisspeptin System throughout the Estrous Cycle in Gilts
Kisspeptin
has been demonstrated to affect reproductive cyclicity and the attainment of
puberty in multiple species, presumably through its actions on gonadotropin
releasing hormone and luteinizing hormone. Kisspeptin administration causes increased
plasma concentrations of LH in pigs, sheep, and rats. The objective of this
experiment was to evaluate changes in the hypothalamic kisspeptin system
throughout the estrous cycle in gilts. Estrus was synchronized in forty
crossbred gilts (191 d, 121 kg) and estrus detection was performed by exposing
gilts to a mature boar. The first day gilts stood immobile was denoted d 1 of
the estrous cycle. Blood samples were collected via jugular venipuncture on d
1, 4, 7, 9, 14, 16, and 19 of the estrous cycle. Ten animals were slaughtered
on d 1, 9, 14, and 21 of the estrous cycle when medial basal hypothalami,
anterior pituitary glands, and blood were collected. Relative expression of
hypothalamic kisspeptin (KISS1), kisspeptin receptor (KISS1R), estrogen receptors-a,
anterior pituitary gland GnRH receptor, β-actin, and GAPDH was determined
using real-time reverse transcriptase PCR. Fold changes in relative expression
were determined using the Relative Expression Software Tool. Relative
expression of KISS1 was increased (P = 0.006) 3.2 fold on d 1 versus d
21 and 2.3 fold (P = 0.003) on d 9 versus d 21 of the estrous cycle, but
was not different (P > 0.05) among the remaining days of the estrous
cycle. Relative expression of estrogen receptor-b was decreased (P =
0.05) 0.8 fold on d 9 versus d 21 and (P = 0.005) 0.7 fold on d 14
versus d 21, but was not different (P > 0.05) among the remaining
days. Relative expression of anterior pituitary gland GnRH receptor was
increased (P