Yan Bin , Wang Jingbo , Zhang Hong , Tian Guoqing , Liu Yuqin
{"title":"淫羊藿苷对高糖培养海马神经元凋亡的影响","authors":"Yan Bin , Wang Jingbo , Zhang Hong , Tian Guoqing , Liu Yuqin","doi":"10.1016/S0254-6272(18)30887-2","DOIUrl":null,"url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate effect of icariin on apoptosis in hippocampal neurons cultured in high glucose, and the possible mechanism behind the action.</p></div><div><h3>METHODS</h3><p>Hippocampus was obtained from newborn 24 h Sprague-Dawley rats and then primarily cultured. Then hippocampal neurons were divided into normal control group, high glucose group, icariin group, icariin + protein kinase B (Akt) inhibitor group and Akt agonist group. After each group was cultured in different conditioned medium for 72 h, we detected the apoptosis of neurons with flow cytometry, and the expression of Akt, p-Akt, B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein (Bax) with western blot.</p></div><div><h3>RESULTS</h3><p>Compared with the normal control group, the apoptosis rate of hippocampal neurons increased significantly (<em>P</em> < 0.01), and p-Akt/Akt and Bcl-2/Bax decreased significantly (<em>P</em> < 0.01) in high glucose group. Compared with the high glucose group, the apoptosis rate of hippocampal neurons decreased significantly (<em>P</em> < 0.01), and p-Akt/Akt and Bcl-2/Bax increased significantly (<em>P</em> < 0.05) in icariin group and Akt agonist group. Compared with the icariin + Akt inhibitor group, the apoptosis rate of hippocampal neurons decreased significantly (<em>P</em> < 0.01), and p-Akt/Akt and Bcl-2/Bax increased significantly (<em>P</em> < 0.01) in icariin group.</p></div><div><h3>CONCLUSION</h3><p>Icariin could reduce the apoptosis of neurons cultured in high glucose, which may be achieved by increasing the phosphorylation of Akt protein in Akt signal pathway, then increasing the expression of Bcl-2 and inhibiting the expression of Bax.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 4","pages":"Pages 556-561"},"PeriodicalIF":2.0000,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30887-2","citationCount":"2","resultStr":"{\"title\":\"Effect of icariin on apoptosis in hippocampal neurons cultured in high glucose\",\"authors\":\"Yan Bin , Wang Jingbo , Zhang Hong , Tian Guoqing , Liu Yuqin\",\"doi\":\"10.1016/S0254-6272(18)30887-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>OBJECTIVE</h3><p>To investigate effect of icariin on apoptosis in hippocampal neurons cultured in high glucose, and the possible mechanism behind the action.</p></div><div><h3>METHODS</h3><p>Hippocampus was obtained from newborn 24 h Sprague-Dawley rats and then primarily cultured. Then hippocampal neurons were divided into normal control group, high glucose group, icariin group, icariin + protein kinase B (Akt) inhibitor group and Akt agonist group. After each group was cultured in different conditioned medium for 72 h, we detected the apoptosis of neurons with flow cytometry, and the expression of Akt, p-Akt, B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein (Bax) with western blot.</p></div><div><h3>RESULTS</h3><p>Compared with the normal control group, the apoptosis rate of hippocampal neurons increased significantly (<em>P</em> < 0.01), and p-Akt/Akt and Bcl-2/Bax decreased significantly (<em>P</em> < 0.01) in high glucose group. Compared with the high glucose group, the apoptosis rate of hippocampal neurons decreased significantly (<em>P</em> < 0.01), and p-Akt/Akt and Bcl-2/Bax increased significantly (<em>P</em> < 0.05) in icariin group and Akt agonist group. Compared with the icariin + Akt inhibitor group, the apoptosis rate of hippocampal neurons decreased significantly (<em>P</em> < 0.01), and p-Akt/Akt and Bcl-2/Bax increased significantly (<em>P</em> < 0.01) in icariin group.</p></div><div><h3>CONCLUSION</h3><p>Icariin could reduce the apoptosis of neurons cultured in high glucose, which may be achieved by increasing the phosphorylation of Akt protein in Akt signal pathway, then increasing the expression of Bcl-2 and inhibiting the expression of Bax.</p></div>\",\"PeriodicalId\":17513,\"journal\":{\"name\":\"Journal of Traditional Chinese Medicine\",\"volume\":\"38 4\",\"pages\":\"Pages 556-561\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2018-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30887-2\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Traditional Chinese Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0254627218308872\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Traditional Chinese Medicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0254627218308872","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
Effect of icariin on apoptosis in hippocampal neurons cultured in high glucose
OBJECTIVE
To investigate effect of icariin on apoptosis in hippocampal neurons cultured in high glucose, and the possible mechanism behind the action.
METHODS
Hippocampus was obtained from newborn 24 h Sprague-Dawley rats and then primarily cultured. Then hippocampal neurons were divided into normal control group, high glucose group, icariin group, icariin + protein kinase B (Akt) inhibitor group and Akt agonist group. After each group was cultured in different conditioned medium for 72 h, we detected the apoptosis of neurons with flow cytometry, and the expression of Akt, p-Akt, B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein (Bax) with western blot.
RESULTS
Compared with the normal control group, the apoptosis rate of hippocampal neurons increased significantly (P < 0.01), and p-Akt/Akt and Bcl-2/Bax decreased significantly (P < 0.01) in high glucose group. Compared with the high glucose group, the apoptosis rate of hippocampal neurons decreased significantly (P < 0.01), and p-Akt/Akt and Bcl-2/Bax increased significantly (P < 0.05) in icariin group and Akt agonist group. Compared with the icariin + Akt inhibitor group, the apoptosis rate of hippocampal neurons decreased significantly (P < 0.01), and p-Akt/Akt and Bcl-2/Bax increased significantly (P < 0.01) in icariin group.
CONCLUSION
Icariin could reduce the apoptosis of neurons cultured in high glucose, which may be achieved by increasing the phosphorylation of Akt protein in Akt signal pathway, then increasing the expression of Bcl-2 and inhibiting the expression of Bax.
期刊介绍:
Journal of Traditional Chinese Medicine(JTCM) is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.