利用豆浆微生物分离物制备益生菌酸奶抑菌及抗氧化活性研究

J NyaE., I. Uffia
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引用次数: 1

摘要

本研究旨在研究利用豆浆微生物分离物制备的益生菌酸奶对细菌的抑制作用和抗氧化活性。采用圆盘扩散法评价了酸奶对金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌和荧光假单胞菌的抑菌活性。该酸奶对所有微生物均有一定的抑制作用。采用二苯基-1-苦味基羟基(DPPH)自由基清除活性、脂质过氧化物酶测定和丁基羟基异醇(BHA)自由基清除活性三种不同的方法测定了酸奶的抗氧化活性。结果表明,与BHA相比,益生菌酸奶具有较高的抗氧化活性。然而,这项研究表明,益生菌酸奶对所有被测试的细菌都有一定的抑制活性,也可以被认为是最有前途的功能食品之一,因为它们具有抗氧化活性和防止氧化损伤和衰老的能力。
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Bacterial Inhibition and Antioxidant Activity of Probiotic Yoghurt Developed Using Microbial Isolates from Soymilk
The aim of this study was to investigate the bacterial inhibition and antioxidant activities of probiotic yoghurts developed using microbial isolates from Soymilk. Bacterial inhibitory activity of yoghurt was evaluated against Staphyloccocus aureus, Bacillus subtillis, Escherichia coli and Pseudomonas flourescens by using the disk diffusion method. The yoghurt showed some inhibitory activities against all the organisms tested. Antioxidant activity of yoghurt was measured using three different methods- Diphenyl-1-picryl hydroxyl (DPPH) radical scavenging activity, lipid peroxidase assay and hydroxyl radical scavenging activity against Butylated hydroxyanisole (BHA) as standard. The results showed that probiotic yoghurt exhibits relative high antioxidant activities as compare to BHA. However, by implications this study suggests that probiotic yoghurt has some inhibitory activities against all the bacteria tested, also can be consider among the most promising functional foods for their antioxidant activity and ability to prevent oxidative damage and attendant ageing.
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