印度尼西亚巴厘地区甘薯C型病毒的分子鉴定

Listihani Listihani, D. G. W. Selangga
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引用次数: 3

摘要

对巴厘省的几个甘薯种植地进行了调查。调查发现,许多植物都表现出斑病症状,如黄化斑点。本研究旨在确定巴厘岛甘薯植物上引起这些症状的病毒的发病率、检测和鉴定。在巴厘岛每个地点(登巴萨、吉安雅、巴东、布莱棱、塔巴南、昆宫、卡兰加森、珍布拉纳、邦利)对10株植物进行有目的取样。根据现场的病毒症状观察到疾病内部。采用Potyvirus通用引物进行核酸鉴定和DNA测序。邦利、布楞和登巴萨县的发病率为50%。RT-PCR和CiFor/CiRev Potyvirus通用引物成功扩增了Bangli所有样本的CI基因±700 bp,而其他8个地区的样本未使用相同引物扩增。甘薯SPVC分离株与东帝汶甘薯分离株(MF572066)核苷酸和氨基酸同源性分别为96.8%和97.4%,为“亚洲”菌株。这表明SPVC已在东爪哇和巴厘岛蔓延。
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Molecular Identification of Sweet potato virus C on Sweetpotato in Bali, Indonesia
A survey was conducted in several sweet potato cultivations in Bali Province. Survey found that many plants exhibited potyvirus symptom, such as chlorosis blotches. This study was to determine disease incidence, detection and identification of the virus causing these symptoms on sweet potato plants in Bali. Samples were collected by purposive sampling of 10 plants from each location in Bali (Denpasar, Gianyar, Badung, Buleleng, Tabanan, Klungkung, Karangasem, Jembrana, Bangli). Disease insidence was observed based on viral symptoms in the field. Identification of nucleic acids was done using Potyvirus universal primer and DNA sequencing. Disease incidence in Bangli, Buleleng, and Denpasar Regencies was > 50%. RT-PCR and CiFor/CiRev Potyvirus universal primers successfully amplified ± 700 bp of CI genes from all samples from Bangli, while samples from 8 other districts were not amplified using the same primers. The SPVC isolate of sweet potato showed nucleotide and amino acid homology similarities with the sweet potato isolate from East Timor (MF572066), 96.8% and 97.4%, respectively and these were referred to the "Asian" strain. This indicates that SPVC has spread in East Java and Bali.
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发文量
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审稿时长
12 weeks
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