{"title":"kindin -1对神经性疼痛大鼠脊髓星形细胞活化的影响","authors":"Bai-song Zhao, Yongying Pan","doi":"10.3760/CMA.J.ISSN.1673-4378.2020.01.001","DOIUrl":null,"url":null,"abstract":"Objective \nTo investigate the effect of kindlin-1 on astrocyte activation in the spinal cord of rats with neuropathic pain. \n \n \nMethods \nA total of 18 male 10-12-week-old SD rats, weight 250-280 g, were divided into three groups according to the random number table method (n=6): a sham operation group (group S), a neuropathic pain group (group NP) and a kindlin-1 inhibition group (group K). A neuropathic pain model was established by chronic constriction of the sciatic nerve. The sciatic nerve of group S was only being exposed without ligation. Rats in group K were intrathecally injected with short hairpin RNA (shRNA) 21 d before surgery to inhibit the expression of kindlin-1. Those in groups S and NP were intrathecally injected with viral vector 21 d before surgery for control. The paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) were measured 1 d before surgery and 1, 4, 7, 10 d and 13 d after surgery, respectively. When pain threshold measurement finished 13 d after surgery, the rats were sacrificed to collect the spinal cord tissue. The expression of kindlin-1 and astrocytes were detected by immunofluorescent double staining. Also, the expression of kindlin-1 in the spinal cord was measured by Western blot. \n \n \nResults \nCompared with group S, rats in group NP had reduced PWMT and shortened PWTL as well as up-regulated expression of kindlin-1 protein in the spinal cord 4, 7, 10 d and 13 d after surgery. Meanwhile, increases in optical density (D) value of kindlin-1 and astrocyte activation rate were also seen in group NP (P<0.05). Compared with group NP, group K had increased PWMT and extended PWTL as well as down-regulated expression of kindlin-1 in the spinal cord 4, 7, 10 d and 13 d after surgery. Meanwhile, decreases in D value of kindlin-1 and astrocyte activation rate were seen in group K (P<0.05). \n \n \nConclusions \nKindlin-1 is involved in the activation of astrocytes in the spinal cord of rats with neuropathic pain. \n \n \nKey words: \nKindlin-1; Neuropathic pain; Astrocyte; Spinal cord","PeriodicalId":13847,"journal":{"name":"国际麻醉学与复苏杂志","volume":"41 1","pages":"1-5"},"PeriodicalIF":0.0000,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Influence of kindlin-1 on astrocyte activation in the spinal cord of rats with neuropathic pain\",\"authors\":\"Bai-song Zhao, Yongying Pan\",\"doi\":\"10.3760/CMA.J.ISSN.1673-4378.2020.01.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective \\nTo investigate the effect of kindlin-1 on astrocyte activation in the spinal cord of rats with neuropathic pain. \\n \\n \\nMethods \\nA total of 18 male 10-12-week-old SD rats, weight 250-280 g, were divided into three groups according to the random number table method (n=6): a sham operation group (group S), a neuropathic pain group (group NP) and a kindlin-1 inhibition group (group K). A neuropathic pain model was established by chronic constriction of the sciatic nerve. The sciatic nerve of group S was only being exposed without ligation. Rats in group K were intrathecally injected with short hairpin RNA (shRNA) 21 d before surgery to inhibit the expression of kindlin-1. Those in groups S and NP were intrathecally injected with viral vector 21 d before surgery for control. The paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) were measured 1 d before surgery and 1, 4, 7, 10 d and 13 d after surgery, respectively. When pain threshold measurement finished 13 d after surgery, the rats were sacrificed to collect the spinal cord tissue. The expression of kindlin-1 and astrocytes were detected by immunofluorescent double staining. Also, the expression of kindlin-1 in the spinal cord was measured by Western blot. \\n \\n \\nResults \\nCompared with group S, rats in group NP had reduced PWMT and shortened PWTL as well as up-regulated expression of kindlin-1 protein in the spinal cord 4, 7, 10 d and 13 d after surgery. Meanwhile, increases in optical density (D) value of kindlin-1 and astrocyte activation rate were also seen in group NP (P<0.05). Compared with group NP, group K had increased PWMT and extended PWTL as well as down-regulated expression of kindlin-1 in the spinal cord 4, 7, 10 d and 13 d after surgery. Meanwhile, decreases in D value of kindlin-1 and astrocyte activation rate were seen in group K (P<0.05). \\n \\n \\nConclusions \\nKindlin-1 is involved in the activation of astrocytes in the spinal cord of rats with neuropathic pain. \\n \\n \\nKey words: \\nKindlin-1; Neuropathic pain; Astrocyte; Spinal cord\",\"PeriodicalId\":13847,\"journal\":{\"name\":\"国际麻醉学与复苏杂志\",\"volume\":\"41 1\",\"pages\":\"1-5\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-01-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"国际麻醉学与复苏杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.1673-4378.2020.01.001\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"国际麻醉学与复苏杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1673-4378.2020.01.001","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Influence of kindlin-1 on astrocyte activation in the spinal cord of rats with neuropathic pain
Objective
To investigate the effect of kindlin-1 on astrocyte activation in the spinal cord of rats with neuropathic pain.
Methods
A total of 18 male 10-12-week-old SD rats, weight 250-280 g, were divided into three groups according to the random number table method (n=6): a sham operation group (group S), a neuropathic pain group (group NP) and a kindlin-1 inhibition group (group K). A neuropathic pain model was established by chronic constriction of the sciatic nerve. The sciatic nerve of group S was only being exposed without ligation. Rats in group K were intrathecally injected with short hairpin RNA (shRNA) 21 d before surgery to inhibit the expression of kindlin-1. Those in groups S and NP were intrathecally injected with viral vector 21 d before surgery for control. The paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) were measured 1 d before surgery and 1, 4, 7, 10 d and 13 d after surgery, respectively. When pain threshold measurement finished 13 d after surgery, the rats were sacrificed to collect the spinal cord tissue. The expression of kindlin-1 and astrocytes were detected by immunofluorescent double staining. Also, the expression of kindlin-1 in the spinal cord was measured by Western blot.
Results
Compared with group S, rats in group NP had reduced PWMT and shortened PWTL as well as up-regulated expression of kindlin-1 protein in the spinal cord 4, 7, 10 d and 13 d after surgery. Meanwhile, increases in optical density (D) value of kindlin-1 and astrocyte activation rate were also seen in group NP (P<0.05). Compared with group NP, group K had increased PWMT and extended PWTL as well as down-regulated expression of kindlin-1 in the spinal cord 4, 7, 10 d and 13 d after surgery. Meanwhile, decreases in D value of kindlin-1 and astrocyte activation rate were seen in group K (P<0.05).
Conclusions
Kindlin-1 is involved in the activation of astrocytes in the spinal cord of rats with neuropathic pain.
Key words:
Kindlin-1; Neuropathic pain; Astrocyte; Spinal cord
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