枯草芽孢杆菌SPB1生物表面活性剂的配方基础,以实现低界面张力和高乳化活性:开发具有潜在用途的个人护理产品的生物基配方

IF 1.6 Q3 WATER RESOURCES Water Practice and Technology Pub Date : 2023-04-10 DOI:10.2166/wpt.2023.052
Mouna Bouassida, Mnif Inès, Nada Fourati, D. Ghribi
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引用次数: 0

摘要

由于其多功能特性,如泡沫、乳化和皮肤保湿活性,生物表面活性剂在化妆品工业中有潜在的应用。本文首先研究了从枯草芽孢杆菌SPB1中分离的化学表面活性剂和脂肽生物表面活性剂对护肤霜配方的影响。对所选择的配方(含15%生物表面活性剂)进行了效率测试。它们的理化性质令人满意。实际上,它的扩散能力为23毫米,水活度为0.72,pH值为5。此外,它们的生物活动也很有趣。结果显示,它对革兰氏阳性细菌的抑制区范围为8至15毫米。对二苯基-1-吡啶酰肼(DPPH)具有抗自由基作用,IC50约为68 mg/mL。其次,为了寻找一种高度稳定的SPB1生物表面活性剂配方,采用Box-Behnken (BB)设计对生物表面活性剂、阿拉伯胶(GA)和甘油的组合进行了优化。结果表明,当SPB1 - BioS、甘油和GA的比例分别为72.62、7.5和1.5%时,表面张力(ST)的最佳值为40.18±0.39 mN/m。在此条件下,乳化指数(EI)-24%为81.76±0.14%。
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Basis for the formulation of Bacillus subtilis SPB1 biosurfactant to achieve low interfacial tension and high emulsification activities: development of a bio-based formula with potential use for personal care products
Thanks to their multifunctional properties, such as foaming, emulsifying and skin hydrating activity, biosurfactants are potentially used in the cosmetic industry. In the present work, firstly, skin cream formulations using chemical surfactant agents and lipopeptide biosurfactants isolated from Bacillus subtilis SPB1 were investigated. The efficiency of the selected formula, containing 15% biosurfactant, was tested. Their physicochemical properties were satisfying. In fact, it shows a spreading ability of 23 mm, a water activity of 0.72 and a pH value of 5. In addition, their biological activities were interesting. As a result, it shows a zone of inhibition against Gram-positive bacteria ranging from 8 to 15 mm. Moreover, it presents an antiradical effect against diphenyl-1-picrylhydrazyl (DPPH) with an IC50 of the order of 68 mg/mL. Second, seeking a highly stable SPB1 biosurfactant formulation for an easy commercial trend, a combination of biosurfactant, Gum Arabic (GA) and glycerol was optimized using the Box–Behnken (BB) design. As analysed, the model predicts an optimal value of the surface tension (ST) equal to 40.18 ± 0.39 mN/m when the respective values of the percentages of SPB1 BioS, glycerol and GA are equal to 72.62, 7.5 and 1.5%. Under these conditions, the emulsification index (EI)-24% is of the order of 81.76 ± 0.14%.
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CiteScore
2.30
自引率
6.20%
发文量
136
审稿时长
14 weeks
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