槲皮素处理对缺氧诱导的EA.hy926细胞凋亡、MDA和FGF2水平的影响

IF 0.7 Q4 PHARMACOLOGY & PHARMACY Journal of Reports in Pharmaceutical Sciences Pub Date : 2021-01-01 DOI:10.4103/jrptps.JRPTPS_70_20
C. Ginting, I. Lister, E. Girsang, D. Artie, J. Aviani, W. Widowati
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引用次数: 2

摘要

背景:先兆子痫(PE)是一种妊娠期疾病,其特征是高血压、蛋白尿和母体全身炎症反应增强。PE影响5%至10%的妊娠,仍然是胎儿和产妇发病率和死亡率的主要因素。缺氧的存在与PE有关。炎症是PE发病机制的必要条件。槲皮素属于类黄酮类,已知具有抗氧化和抗炎活性。目的:本研究旨在确定槲皮素在缺氧(2%O2)诱导的人内皮细胞(EA.hy926)系中降低细胞凋亡百分比、脂质过氧化物酶(MDA)和FGF2水平的潜力,作为PE模型。材料和方法:本研究采用五种处理方法(阴性对照、载体对照、缺氧对照、槲皮素25µg/mL和槲皮素6.25µg/mL)测定活细胞、坏死细胞和凋亡细胞的百分比;MDA和FGF2水平对缺氧诱导的内皮细胞作为PE模型。采用ELISA法测定MDA和FGF2的含量。使用流式细胞术方法测量活细胞、坏死细胞和凋亡细胞。结果:与缺氧对照组相比,槲皮素能降低MDA和FGF2水平;增加活细胞百分比;与缺氧对照细胞相比,凋亡和坏死细胞百分比降低。结论:作为PE模型,槲皮素具有抗氧化和抗炎的特性,可以降低缺氧诱导的内皮细胞中凋亡细胞的百分比,抑制MDA和FGF2的水平,并增加活细胞的百分比。
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Apoptotic, MDA, and FGF2 level of quercitrin treatment on hypoxic-induced EA.hy926 Cell Line
Background: Preeclampsia (PE) is pregnancy disorder that is characterized by hypertension, proteinuria, and an enhanced maternal systemic inflammatory response. PE affects 5% to 10% of all pregnancies and remains a leading factor of fetal and maternal morbidity and mortality. The existence of oxygen deprivation is involved in PE. Inflammation is a requisite to the pathogenesis of PE. Quercitrin belongs to the flavonoid group that is known to have antioxidant and anti-inflammatory activity. Aims: This study aims at determining the potential of quercitrin to reduce the percentage of apoptosis, levels of lipid peroxidase (MDA), and FGF2 in the human endothelial cell (EA.hy926) line that is induced by hypoxia (2% O2) as a PE model. Materials and Methods: Five treatments were used in this study (negative control, vehicle control, hypoxia control, quercitrin 25 µg/mL, and quercitrin 6.25 µg/mL) to determine the live, necrotic, and apoptotic cells percentage; MDA and FGF2 levels toward hypoxia-induced endothelial cells as a PE model. ELISA method was used to measure the MDA and FGF2 levels. Live, necrotic, and apoptotic cells were measured by using the flow cytometry method. Result: Quercitrin was capable of decreasing the MDA and FGF2 levels compared with hypoxia control; of increasing live cells percentage; and of decreasing apoptotic and necrotic cells percentage compared with hypoxia control cells. Conclusion: This study showed that quercitrin possesses antioxidant and anti-inflammatory properties that can decrease the percentage of the apoptotic cells, suppress MDA levels and FGF2 levels, and increase live cells percentage in hypoxia-induced endothelial cells as a PE model.
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来源期刊
Journal of Reports in Pharmaceutical Sciences
Journal of Reports in Pharmaceutical Sciences Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
1.40
自引率
0.00%
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0
期刊介绍: The Journal of Reports in Pharmaceutical Sciences(JRPS) is a biannually peer-reviewed multi-disciplinary pharmaceutical publication to serve as a means for scientific information exchange in the international pharmaceutical forum. It accepts novel findings that contribute to advancement of scientific knowledge in pharmaceutical fields that not published or under consideration for publication anywhere else for publication in JRPS as original research article. all aspects of pharmaceutical sciences consist of medicinal chemistry, molecular modeling, drug design, pharmaceutics, biopharmacy, pharmaceutical nanotechnology, pharmacognosy, natural products, pharmaceutical biotechnology, pharmacology, toxicology and clinical pharmacy.
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