番荔枝叶(Annona Muricata L.)作为纳巴蒂真菌生长抑制剂Patogen Colletotrichum Gloeosporioides候选植物的潜力

Potensi Daun, Sirsak Annona, .. MuricataL, sebagai Kandidat, Fungisida Nabati, Penghambat Pertumbuhan, Cendawan Patogen, Colletotrichum Gloeosporioides, Syayyidah Fatimatuz, Lailatul Zahro, R. Putri, Galuh Istiqomah, Citra Cahya, Rohmana Denise Ayu, Christyfani Yustikaningrum, Sindhuwati, Christyfani Sindhuwati
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引用次数: 0

摘要

炭疽菌的侵袭给农民造成了损失。长期使用合成杀菌剂来治疗这种真菌对环境和消费者有害。番荔枝叶(Annona muricata L.)含有抗氧化化合物,对植物害虫具有拒食、接触毒和胃毒的作用,并可能在收获后园艺中作为炭疽菌的生长抑制剂成为蔬菜杀菌剂。本研究采用正己烷、乙酸乙酯、95%乙醇为萃取溶剂的多级萃取法。采用岛津1800紫外-可见分光光度计对番石榴叶提取物中生物碱、黄酮类化合物、皂苷和单宁等次生代谢产物的含量进行了定性和定量测定。以番荔枝叶提取物浸渍一定时间的圆盘纸为实验材料,对番荔枝叶提取物进行了抑菌试验。定性检测结果表明,番荔枝叶提取物中含有生物碱、黄酮类化合物、皂苷和单宁。真菌抑菌区试验结果表明,当提取时间为144小时、含量为50%时,番石榴叶提取物的抑菌区直径最大,为1.7 cm,效果最佳。
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Potensi Daun Sirsak (Annona Muricata L.) sebagai Kandidat Fungisida Nabati Penghambat Pertumbuhan Cendawan Patogen Colletotrichum Gloeosporioides
The attack of the Colletotrichum gloeosporioides fungus causes losses to farmers. The prolonged use of synthetic fungicides to treat this fungus harms the environment and consumers. Soursop leaves (Annona muricata L.) contain antioxidant compounds that act as antifeedants, contact poisons, and stomach poisons for plant pests and can potentially become a vegetable fungicide as a growth inhibitor for Colletotrichum gloeosporioides in postharvest horticulture. This study used a multilevel extraction method with solvents of n-hexane, ethyl acetate, and 95% ethanol. Tests for the content of secondary metabolites of soursop leaf extract in the form of alkaloids, flavonoids, saponins, and tannins were carried out qualitatively and quantitatively using a UV-Vis Shimadzu 1800 spectrophotometer. The inhibition test of the extract against the fungus Colletotrichum gloeosporioides was carried out using disc paper soaked with soursop leaf extract for a certain time. The qualitative test results of soursop leaf extract showed that it contained alkaloids, flavonoids, saponins, and tannins. The fungal inhibition zone test showed the most optimal results on soursop leaf extract with a long extraction time of 144 hours and a content of 50%, indicated by the largest diameter of the inhibition zone against the fungus Colletotrichum gloeosporioides, namely 1.7 cm.
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