牛骨支架材料不同孔径对口腔微生物群的影响

Fangchen Liu, Rui Ma, Yidan Ma, Geng Li, Yongsheng Lin, Bin Ma, Lin Xu, Jianye Zhou
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引用次数: 0

摘要

骨支架是一种骨修复材料,但骨支架感染是一个常见而严重的临床问题,尚未在口腔微生物群的背景下得到很好的研究。我们从成年和胎儿牛松质骨中制备了两种不同孔径的骨支架材料,并将其与羟基磷灰石(HA)粉末进行了比较。培养后,我们利用健康人的唾液促进了口腔微生物群在这三种材料上的初步定植。通过高通量测序和分析,我们发现骨支架材料的不同孔径对口腔微生物群的初始定殖有一定影响,影响β-多样性和功能图谱。冗余分析(RDA)和共现分析表明,口腔微生物群在孔径较小的胎儿骨支架材料中的初始微生物定殖更容易引起感染。此外,骨支架材料中可能存在一些功能性细菌,如芽孢杆菌属和乳杆菌属,它们在口腔菌群的早期定殖中发挥着相互作用。
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The Influence of Different Pore Sizes of Bovine Bone Scaffold Material on the Oral Microbiota
Bone scaffolds are a kind of bone repair material, but bone scaffold infection is a common and serious clinical problem that has not been well studied in the context of the oral microbiota. We prepared two different bone scaffold materials with different pore sizes from adult and fetal bovine cancellous bones and compared them with hydroxyapatite (HA) powder. After incubation, we facilitated the initial colonization by the oral microbiota on the three materials by using the saliva of healthy people. Through high-throughput sequencing and analysis, we found that the different pore sizes of the bone scaffold materials had a certain influence on the initial colonization by the oral microbiota, affecting β-diversity and functional profiles. Redundancy analysis (RDA) and cooccurrence analysis indicated that initial microbial colonization by the oral microbiota in fetal bone scaffold materials with smaller pore sizes was more likely to cause infection. In addition, there may be some functional bacteria that play an interactive role in early colonization by the oral microflora in bone scaffold materials, such as those of the genera Bacillus and Lactobacillus.
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