肉鸡盲肠和有机肉鸡盲肠大肠杆菌分离株的耐药性研究

Septianita Eva Rozani, M. E. Kustyawati, D. Sartika, Subeki Subeki, T. P. Utomo
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摘要

本研究旨在采用Muller-Hinton琼脂碟扩散法测定肉鸡盲肠和有机肉鸡盲肠分离的大肠杆菌的耐药性水平,每组10个盲肠样本。样本采集采用有目的采样法。用吲哚、甲基红Voges-Proskauer (MRVP)和柠檬酸盐(IMVIC)生化试验进一步检测疑似大肠杆菌分离株。进一步对分离的大肠杆菌进行抗生素敏感性试验。肉鸡盲肠大肠杆菌耐药性检测结果显示,对庆大霉素(70%)、氨苄西林(100%)、头孢菌素(100%)、萘啶酸(86%)、链霉素(80%)、红霉素(100%)、土霉素(80%)的耐药水平较高,对氯霉素(30%)的耐药水平最低;有机肉鸡对氨苄西林(100%)、头孢菌素(70%)、红霉素(100%)的耐药水平最低,对庆大霉素(10%)的耐药水平最低;土霉素(20%),氯霉素(10%)从肉鸡和有机肉鸡的盲肠中分离出的大肠杆菌具有较高的耐药性。本研究的结论是,肉鸡盲肠分离的大肠杆菌对除氯霉素类抗生素外的几乎所有抗生素都有较高的耐药性,而有机肉鸡大肠杆菌对除庆大霉素、萘啶酸和氯霉素外的抗生素的耐药性低于肉鸡,但仍有较高的耐药性。
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Antibiotic Resistance of Escherichia coli Isolate from Broiler Cecum and Organic Broiler Cecum
This study aims to determine the level of antibiotic resistance of Escherichia coli bacterial isolates from broiler cecum and organic broiler cecum using the disc diffusion method on Muller-Hinton Agar as many as 10 cecum samples for each group.Collection of samples using purposive sampling method. Isolates suspected of being Escherichia coliwere further tested using Indole, Methyl Red Voges-Proskauer (MRVP), and Citrate (IMVIC) biochemical tests. E. coli isolates were further tested with the Antimicrobial Susceptibility Testing which aims to test antibiotic sensitivity. The results obtained from testing antibiotic resistance against Escherichia coli in broiler caecums showed a fairly high level of resistance to Gentamicin (70%), Ampicillin (100%), Cephalotin (100%), Nalixid Acid (86%), Streptomycin (80%), Erythromycin (100%), Oxytetracycline (80%), and the lowest was the antibiotic Chloramphenicol (30%), while the level of resistance in organic broilers showed Ampicillin (100%), Cefalotin (70%), Erythromycin (100%), the lowest resistance was Gentamicin (10%), Oxytetracycline (20%), Chlorampenicol (10%). Escherichia coli isolates obtained from the caecum of broiler and organic broiler showed a fairly high level of resistance. Conclusions of this study were resistance of Escherichia coli bacteria isolated from broiler cecum showed high results to almost all antibiotics except for chloramphenicol antibiotics, while resistance to Escherichia coli bacteria in organic broilers was lower than broilers but still showed a fairly high level of resistance, except for antibiotics Gentamicin, Nalixid acid , and chloramphenicol.
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