{"title":"氧化石墨烯-低温凝胶三维基质功能化中空纤维膜生物反应器促进肝细胞锚定和功能维持","authors":"Surendra Kumar Verma, Akshay Modi, Jayesh Bellare","doi":"10.1002/mds3.10128","DOIUrl":null,"url":null,"abstract":"<p>The liver support system (bioartificial liver, BAL) is a potentially efficient treatment during reversible acute liver disease or while waiting for a liver transplant. The main challenge in implementing BAL is a bioreactor, which can fulfil the needs of hepatocyte culture, mass transfer and immunobarriers. In the present study, polymer nanocomposite hollow fibre membranes (HFMs) were fabricated using polyethersulphone (P) as a base polymer, vitamin E TPGS (T) as an additive and carboxylated carbon nanotubes (C) as a filler. These PTC HFMs were functionalized with cryogel (Cr), with or without carboxylated graphene oxide (G), known as PTC-Cr or PTC-CrG HFMs, respectively. These HFM substrates were characterized and evaluated for hemocompatibility, cytotoxicity and cellular functionality. Among all the HFM substrates, PTC-CrG HFMs showed superior hemocompatibility and minimal cytotoxicity. Furthermore, a liver cell bioreactor was developed and studied using PTC-CrG HFMs. The goat primary liver cells were seeded in a bioreactor and cultured in continuous mode. The cell functionality was better maintained in the continuous mode as compared to that in the batch culture. The three-dimensional PTC-CrG HFMs-based bioreactor can be used for liver cell bioreactor, bioartificial liver system, drug testing/toxicology metabolic study and mass cell culture.</p>","PeriodicalId":87324,"journal":{"name":"Medical devices & sensors","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/mds3.10128","citationCount":"5","resultStr":"{\"title\":\"Hollow fibre membrane bioreactor functionalized with GO-cryogel 3D matrix promotes liver cell anchoring and their functional maintenance\",\"authors\":\"Surendra Kumar Verma, Akshay Modi, Jayesh Bellare\",\"doi\":\"10.1002/mds3.10128\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The liver support system (bioartificial liver, BAL) is a potentially efficient treatment during reversible acute liver disease or while waiting for a liver transplant. The main challenge in implementing BAL is a bioreactor, which can fulfil the needs of hepatocyte culture, mass transfer and immunobarriers. In the present study, polymer nanocomposite hollow fibre membranes (HFMs) were fabricated using polyethersulphone (P) as a base polymer, vitamin E TPGS (T) as an additive and carboxylated carbon nanotubes (C) as a filler. These PTC HFMs were functionalized with cryogel (Cr), with or without carboxylated graphene oxide (G), known as PTC-Cr or PTC-CrG HFMs, respectively. These HFM substrates were characterized and evaluated for hemocompatibility, cytotoxicity and cellular functionality. Among all the HFM substrates, PTC-CrG HFMs showed superior hemocompatibility and minimal cytotoxicity. Furthermore, a liver cell bioreactor was developed and studied using PTC-CrG HFMs. The goat primary liver cells were seeded in a bioreactor and cultured in continuous mode. The cell functionality was better maintained in the continuous mode as compared to that in the batch culture. The three-dimensional PTC-CrG HFMs-based bioreactor can be used for liver cell bioreactor, bioartificial liver system, drug testing/toxicology metabolic study and mass cell culture.</p>\",\"PeriodicalId\":87324,\"journal\":{\"name\":\"Medical devices & sensors\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-09-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/mds3.10128\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Medical devices & sensors\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/mds3.10128\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medical devices & sensors","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/mds3.10128","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
摘要
含肝细胞的体外生物人工肝(extracorporeal bioartificial liver, BAL)支持系统是急性肝衰竭患者辅助肝移植或促进肝脏再生的治疗方法之一(Zhang et al., 2014)。改善BAL的主要特点包括细胞来源、维持肝细胞功能和高效的生物反应器设计。人类肝细胞的稀缺性及其在体外增殖能力的有限性吸引了更好的细胞培养环境,可以改善原代细胞扩增、永生化细胞系或干细胞的干细胞分化。因此,肝细胞微环境的综合特征和生物反应器的设计可以产生有效和可扩展的以肝脏为中心的功能(Allen & Bhatia, 2002)。在组织工程中,维持功能细胞的三维(3D)基质是一种潜在的体外仿生系统。体外系统优于体内模型,因为它具有以下优点:评估毒性机制,分析药物反应关系,只需少量药物即可进行直接筛选,成本较低,节省时间。免疫分离也是BAL的一个重要方面。有不同的免疫分离方法,每种方法都有其优点
Hollow fibre membrane bioreactor functionalized with GO-cryogel 3D matrix promotes liver cell anchoring and their functional maintenance
The liver support system (bioartificial liver, BAL) is a potentially efficient treatment during reversible acute liver disease or while waiting for a liver transplant. The main challenge in implementing BAL is a bioreactor, which can fulfil the needs of hepatocyte culture, mass transfer and immunobarriers. In the present study, polymer nanocomposite hollow fibre membranes (HFMs) were fabricated using polyethersulphone (P) as a base polymer, vitamin E TPGS (T) as an additive and carboxylated carbon nanotubes (C) as a filler. These PTC HFMs were functionalized with cryogel (Cr), with or without carboxylated graphene oxide (G), known as PTC-Cr or PTC-CrG HFMs, respectively. These HFM substrates were characterized and evaluated for hemocompatibility, cytotoxicity and cellular functionality. Among all the HFM substrates, PTC-CrG HFMs showed superior hemocompatibility and minimal cytotoxicity. Furthermore, a liver cell bioreactor was developed and studied using PTC-CrG HFMs. The goat primary liver cells were seeded in a bioreactor and cultured in continuous mode. The cell functionality was better maintained in the continuous mode as compared to that in the batch culture. The three-dimensional PTC-CrG HFMs-based bioreactor can be used for liver cell bioreactor, bioartificial liver system, drug testing/toxicology metabolic study and mass cell culture.