植物生长促进菌（PGPB）：PGP活性的分离和筛选。

Q1 Agricultural and Biological Sciences Current protocols in plant biology Pub Date : 2018-02-13 DOI:10.1002/pb.20054

Adriana Ambrosini, Luciane M. P. Passaglia

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引用次数: 23

摘要

植物根系与许多不同类型的有益微生物和病原微生物有关。植物生长促进（根）细菌（PGPB或PGPR）是从世界各地的植物作物中分离出来的，其中许多被用作农业接种剂。农业生物肥料化和病原体的生物控制是化学使用的环保替代品，能源、环境和经济成本更低。PGPB的分离和评估是确定能够改善植物发育和生产力的细菌的基本步骤。在本单元中，我们提出了从土壤和植物根中分离细菌的方案（“推定的”重氮营养细菌和内孔形成细菌），并评估它们对促进植物生长的一些有益特性（例如，固氮、吲哚化合物和铁载体的产生、磷酸盐溶解和1-氨基环丙烷-1-羧酸脱氨酶活性）。©2017 John Wiley&Sons，股份有限公司版权所有。

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Plant Growth–Promoting Bacteria (PGPB): Isolation and Screening of PGP Activities

Plant roots are associated with numerous and diverse types of beneficial and pathogenic microorganisms. Plant growth–promoting (rhizo)bacteria (PGPB or PGPR) are isolated from plants crops worldwide, and many of them are used as agricultural inoculants. Agricultural biofertilization and biocontrol of pathogens are eco-friendly alternatives to chemical usage and have less energy, environmental, and economic costs. PGPB isolation and evaluation are essentials steps for determining bacteria that are able to improve plant development and productivity. In this unit, we present protocols to isolate bacteria from soil and plant roots (“putative” diazotrophic and endospore-forming bacteria), as well to evaluate some of their beneficial characteristics for the promotion of plant growth (e.g., nitrogen fixation, production of indolic compounds and siderophores, phosphate solubilization, and 1-aminocyclopropane-1-carboxylate deaminase activity). © 2017 by John Wiley & Sons, Inc.

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Current protocols in plant biology Agricultural and Biological Sciences-Plant Science

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. Current Protocols in Plant Biology provides reproducible step-by-step instructions for protocols relevant to plant research. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols in Plant Biology to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Plant Biology is the comprehensive source for protocols in the multidisciplinary field of plant biology, providing an extensive range of protocols from basic to cutting edge. Coverage includes: Extraction and analysis of DNA, RNA, proteins Chromosome analysis Transcriptional analysis Protein expression Metabolites Plant enzymology Epigenetics Plant genetic transformation Mutagenesis Arabidopsis, Maize, Poplar, Rice, and Soybean, and more.