Comportamiento fisiológico de pollos de engorde usando dietas con Tithonia diversifolia y

Probiotics allow establishing and controlling the beneficial microflora and gradually reduce the pathogenic microflora. Tithonia diversifola (TD) is an alternative to replace protein from other more expensive sources in diets for poultry; therefore, the treatments used TD replacements with 0, 5, 10 y 15% with probiotics (WP) and without probiotics (W/OP) addition: Saccharomyces cerevisiae (SC), Lactobacillus Acidophilus (LA) and Bacillus subtilis (BS) and the mix of these (MP) for estimating the in-vivo digestibility. 300 Cobb avian chickens were used, with an average weight of 453.4 ± 24 g randomly distributed with a factorial arrangement 4x5x3x5. Digestibility of dry matter (DM), crude protein (CP) and fiber (CF) was determined through the total feces collection method; additionally: Hemogram (CBC) and blood chemistry (BC) were analyzed. DM digestibility increased with the proportion of TD (15%) in the diet and reached 68% W/OP; while this variable rose to 70.67% with SC (P<0.05). Digestibility of CP and CF came up to 85.6 and 82.6% with 15% TD and LA respectively. In WP treatments the red blood cell count (RBC) increased by BS; medium corpuscular volume (MCV), concentration of mean corpuscular hemoglobin (MCHC), and platelet count with LA and MP; and eosinophils with SC; while the total count of white blood cells (WBC) decreased in all the treatments with regard to TD 0%, just like the monocyte differential (MD) with MP (P<0.05). The effect of TD was lower in hemoglobin (Hb) and the RBC with TD 5%, and MD with TD 10 and 15% (P<0.05). Cholesterol decreased with SC and MP, while triglycerides (TG) increased in all the treatments with regard to TD 0%. Aspartate aminotransferase (AST), total protein, sodium and potassium were modified by the probiotics, being this effect lower with TD; triglycerides, uric acid and sodium were affected with the three levels of TD replacement (P<0.05). We can conclude that the inclusion of TD from 10% in combination with probiotics in the diet of broilers, improves its digestibility, likewise that the CBC and BC parameters showed modifications, which allows assessing the nutritional behavior of the diet and providing guidance on an appropriate balance.