The effect of mass cultured Phronima pacifica feed using Caulerpa lentillifera substrate on growth performance and nutritional quality of pacific white shrimp (Litopenaeus vannamei)

ABSTRACT The nutritional content of P. pacifica for protein ranges from 40% to 50% fat 10–15% and EPA is 7.52% not much different from the nutritional content of Artemia sp. i.e. 45–55%, fat 10–12%. The aim of the study was to find of the effect of different levels of substrate (C. lentillifera) in the culture of P. pacifica resulting in the best growth performance and nutritional quality of L. vannamei. The L. vannamei used, weighing 0.029 ± 0.001 g were stocked at a density of 5 individual L-1 and reared for 30 days. Treatment of Pacific white shrimp larvae feeding with P. pacifica resulted from mass culture using a substrate the P. pacifica cultured at different levels of C. lentillifera as a substrate: A (0 g m−2); B (20 g m−2); C (40 g m−2); and D (60 g m−2). The results showed that the best treatment of pacific white shrimp fed by P. pacifica cultured using substrate C. lentillifera 60 g m−2 (D). The results showed that post-larvae of L. vannamei shrimp fed by P. pacifica cultured using the substrate of C. lentillifera 60 gram/m2 (D) produces the highest value on RGR of 34.98%, the absolute length growth of 1.97 cm; biomass weight of 0.0104 g; the survival rate of 96% and grazing rate 23.034 individual day−1. 62.15% of protein and 8.04% of fat; the EPA was 7.95%, and lysine was 11.95%.