聚(D, l -丙交酯)/聚乙二醇混合膜用于角质细胞培养和皮肤重建

IF 3.9 3区医学 Q2 ENGINEERING, BIOMEDICAL Biomedical materials Pub Date : 2019-09-09 DOI:10.1088/1748-605X/ab3aa2

Y. Nashchekina, I. Samusenko, I. Zorin, L. Kukhareva, A. Bilibin, M. Blinova

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引用次数: 5

摘要

本研究的目的是开发一种新型多孔薄聚(D, l -丙交酯)(PLA)薄膜，作为用于替代受损皮肤的角质形成细胞的组织工程支架。采用旋涂技术制备了聚乳酸/聚乙二醇共混膜(PEG: Mw: 6000或15000)。研究了这些共混膜的性能和结构。经水培养后，用微纤维胶原蛋白修饰PDLA/PEG(6000)共混膜，增加亲水性，改善角质细胞粘附。将原代角质形成细胞播种于聚乳酸膜上，培养9 d后移植于模型皮肤缺损创面大鼠。采用组织学和免疫化学方法观察角化细胞移植后创面愈合情况。发现角质形成细胞移植于胶原修饰的多孔聚乳酸膜上后，皮肤损伤恢复效果最好。

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Poly(D,L-lactide)/PEG blend films for keratinocyte cultivation and skin reconstruction

The objective of this study was to develop a novel porous thin poly(D,L-lactide) (PLA) film as a tissue-engineering scaffold for keratinocytes used for the replacement of damaged skin. Poly(D,l-lactic acid)/poly(ethylene glycol) (PEG: Mw 6000 or 15 000) blend films were formed by a spin coating technique. The properties and structures of these blend films were investigated. PDLA/PEG (6000) blend films were modified by microfibrillar collagen after water incubation to increase hydrophilicity and improve keratinocyte adhesion. Primary keratinocytes were seeded on PLA films, cultivated for 9 d and transplanted to rats with a model skin defect wound. The wound’s healing after keratinocyte transplantation was assayed with histological and immunochemical methods. It was found that skin damage recovery was the most effective after transplantation of keratinocytes on porous PLA film modified with collagen.

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来源期刊

Biomedical materials 工程技术-材料科学：生物材料

CiteScore

6.70

自引率

7.50%

发文量

294

审稿时长

3 months

期刊介绍： The goal of the journal is to publish original research findings and critical reviews that contribute to our knowledge about the composition, properties, and performance of materials for all applications relevant to human healthcare. Typical areas of interest include (but are not limited to): -Synthesis/characterization of biomedical materials- Nature-inspired synthesis/biomineralization of biomedical materials- In vitro/in vivo performance of biomedical materials- Biofabrication technologies/applications: 3D bioprinting, bioink development, bioassembly & biopatterning- Microfluidic systems (including disease models): fabrication, testing & translational applications- Tissue engineering/regenerative medicine- Interaction of molecules/cells with materials- Effects of biomaterials on stem cell behaviour- Growth factors/genes/cells incorporated into biomedical materials- Biophysical cues/biocompatibility pathways in biomedical materials performance- Clinical applications of biomedical materials for cell therapies in disease (cancer etc)- Nanomedicine, nanotoxicology and nanopathology- Pharmacokinetic considerations in drug delivery systems- Risks of contrast media in imaging systems- Biosafety aspects of gene delivery agents- Preclinical and clinical performance of implantable biomedical materials- Translational and regulatory matters