禽分枝杆菌亚种的分子分型。哥伦比亚安蒂奥基亚奶牛场的副结核

Pub Date : 2020-08-13 DOI:10.21897/rmvz.1816

N. M. Correa-Valencia, Roberto Damián Moyano, M. Romano, J. A. Fernández-Silva

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引用次数: 3

摘要

目标。目的:测定禽分枝杆菌亚种。哥伦比亚奶牛群环境样本中的副结核(MAP)分子多样性。材料和方法。选取25头IS900-qPCR map阳性奶牛的环境样品，在含有mycoblin J的Herrold’s蛋黄培养基中重复培养，获得分离菌株。可疑菌落经MAPIS900-qPCR确认。采用分枝杆菌穿插重复单位-可变数目串联重复(MIRU-VNTR)和多位点短序列重复(MLSSR)技术对阳性DNA进行分型，分析分离株间的遗传差异。结果。MIRU-VNTR分型显示两种不同的基因型(INMV 2和INMV 36)。采用MLSSR技术，提高了玉米的抗氧化能力

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Tipificación molecular de Mycobacterium avium subsp. paratuberculosis en lecherías de Antioquia, Colombia

Objective. To determine Mycobacterium avium subsp. paratuberculosis (MAP) molecular diversity in environmental samples from Colombian dairy herds. Materials and methods. Environmental samples from 25 IS900-qPCR MAP-positive dairy herds were cultured by duplicate in Herrold ́s egg yolk medium with mycobactin J to obtain isolates. Suspicious colonies were confirmed by MAPIS900-qPCR. Positive DNA was sub-typed using mycobacterial interspersed repetitive units-variable number of tandem repeat (MIRU-VNTR) and multilocus short sequence repeats (MLSSR) techniques to analyze the genetic differences between the isolates. Results. Sub-typing revealed two different genotypes by MIRU-VNTR (INMV 2 and INMV 36). MLSSR technique was carried out to increase the

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