{"title":"原发性高血压患者外周血中microRNA-126、microRNA-155、Klotho蛋白、TGF-β1蛋白的表达及临床意义","authors":"Junchong Chen","doi":"10.3760/CMA.J.ISSN.1008-6706.2020.07.007","DOIUrl":null,"url":null,"abstract":"Objective \nTo investigate the expression of microRNA-126, microRNA-155, Klotho protein and transforming growth factor-β1 (TGF-β1) in peripheral blood of patients with essential hypertension. \n \n \nMethods \nFrom December 2015 to December 2018, 80 patients with essential hypertension admitted to Lishui Central Hospital were selected in the study, and 80 healthy people from Lishui Central Hospital from December 2015 to December 2018 were selected as the control group.The expressions of microRNA-126 and microRNA-155 were detected by RT-PCR, and the expressions of Klotho and TGF-β1 were detected by ELISA.The changes of systolic and diastolic blood pressure, the expressions of microRNA-126 and microRNA-155 in peripheral blood, Klotho protein and TGF-β1 protein were compared between the two groups. \n \n \nResults \nThe expression of microRNA-126 (0.23±0.07) and microRNA-155 (0.84±0.14) in peripheral blood of the hypertension group was lower than that of the control group [(1.16±0.24) and (1.37±0.21)] (t=33.273, 18.782, all P<0.05). The serum levels of Klotho protein in the hypertension group[(123.42±9.47)ng/L] was lower than that in the control group [(143.56±14.68)ng/L], while the serum level of TGF-β1 protein the hypertension group[(561.32±58.39)ng/L] was higher than that in the control group [(408.97±25.13)ng/L] (t=10.312, 21.436, allP<0.05). \n \n \nConclusion \nThe expression of microRNA-126 and microRNA-155 in peripheral blood of patients with essential hypertension is down-regulated, and the expression of Klotho protein and TGF-β1 protein is up-regulated, which has certain guiding significance for clinical diagnosis and treatment. \n \n \nKey words: \nHypertension; MicroRNA-126; MicroRNA-155; Klotho protein; Transforming growth factor β1","PeriodicalId":10226,"journal":{"name":"中国基层医药","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Expression and clinical significance of microRNA-126, microRNA-155, Klotho protein and TGF-β1 protein in peripheral blood of patients with essential hypertension\",\"authors\":\"Junchong Chen\",\"doi\":\"10.3760/CMA.J.ISSN.1008-6706.2020.07.007\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective \\nTo investigate the expression of microRNA-126, microRNA-155, Klotho protein and transforming growth factor-β1 (TGF-β1) in peripheral blood of patients with essential hypertension. \\n \\n \\nMethods \\nFrom December 2015 to December 2018, 80 patients with essential hypertension admitted to Lishui Central Hospital were selected in the study, and 80 healthy people from Lishui Central Hospital from December 2015 to December 2018 were selected as the control group.The expressions of microRNA-126 and microRNA-155 were detected by RT-PCR, and the expressions of Klotho and TGF-β1 were detected by ELISA.The changes of systolic and diastolic blood pressure, the expressions of microRNA-126 and microRNA-155 in peripheral blood, Klotho protein and TGF-β1 protein were compared between the two groups. \\n \\n \\nResults \\nThe expression of microRNA-126 (0.23±0.07) and microRNA-155 (0.84±0.14) in peripheral blood of the hypertension group was lower than that of the control group [(1.16±0.24) and (1.37±0.21)] (t=33.273, 18.782, all P<0.05). The serum levels of Klotho protein in the hypertension group[(123.42±9.47)ng/L] was lower than that in the control group [(143.56±14.68)ng/L], while the serum level of TGF-β1 protein the hypertension group[(561.32±58.39)ng/L] was higher than that in the control group [(408.97±25.13)ng/L] (t=10.312, 21.436, allP<0.05). \\n \\n \\nConclusion \\nThe expression of microRNA-126 and microRNA-155 in peripheral blood of patients with essential hypertension is down-regulated, and the expression of Klotho protein and TGF-β1 protein is up-regulated, which has certain guiding significance for clinical diagnosis and treatment. \\n \\n \\nKey words: \\nHypertension; MicroRNA-126; MicroRNA-155; Klotho protein; Transforming growth factor β1\",\"PeriodicalId\":10226,\"journal\":{\"name\":\"中国基层医药\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中国基层医药\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.1008-6706.2020.07.007\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国基层医药","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1008-6706.2020.07.007","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Expression and clinical significance of microRNA-126, microRNA-155, Klotho protein and TGF-β1 protein in peripheral blood of patients with essential hypertension
Objective
To investigate the expression of microRNA-126, microRNA-155, Klotho protein and transforming growth factor-β1 (TGF-β1) in peripheral blood of patients with essential hypertension.
Methods
From December 2015 to December 2018, 80 patients with essential hypertension admitted to Lishui Central Hospital were selected in the study, and 80 healthy people from Lishui Central Hospital from December 2015 to December 2018 were selected as the control group.The expressions of microRNA-126 and microRNA-155 were detected by RT-PCR, and the expressions of Klotho and TGF-β1 were detected by ELISA.The changes of systolic and diastolic blood pressure, the expressions of microRNA-126 and microRNA-155 in peripheral blood, Klotho protein and TGF-β1 protein were compared between the two groups.
Results
The expression of microRNA-126 (0.23±0.07) and microRNA-155 (0.84±0.14) in peripheral blood of the hypertension group was lower than that of the control group [(1.16±0.24) and (1.37±0.21)] (t=33.273, 18.782, all P<0.05). The serum levels of Klotho protein in the hypertension group[(123.42±9.47)ng/L] was lower than that in the control group [(143.56±14.68)ng/L], while the serum level of TGF-β1 protein the hypertension group[(561.32±58.39)ng/L] was higher than that in the control group [(408.97±25.13)ng/L] (t=10.312, 21.436, allP<0.05).
Conclusion
The expression of microRNA-126 and microRNA-155 in peripheral blood of patients with essential hypertension is down-regulated, and the expression of Klotho protein and TGF-β1 protein is up-regulated, which has certain guiding significance for clinical diagnosis and treatment.
Key words:
Hypertension; MicroRNA-126; MicroRNA-155; Klotho protein; Transforming growth factor β1
期刊介绍：
Since its inception, the journal "Chinese Primary Medicine" has adhered to the development strategy of "based in China, serving the grassroots, and facing the world" as its publishing concept, reporting a large amount of the latest medical information at home and abroad, prospering the academic field of primary medicine, and is praised by readers as a medical encyclopedia that updates knowledge. It is a core journal in China's medical and health field, and its influence index (CI) ranks Q2 in China's academic journals in 2022. It was included in the American Chemical Abstracts in 2008, the World Health Organization Western Pacific Regional Medical Index (WPRIM) in 2009, and the Japan Science and Technology Agency Database (JST) and Scopus Database in 2018, and was included in the Wanfang Data-China Digital Journal Group and the China Academic Journal Comprehensive Evaluation Database.
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