健脾益气阻血方对辛伐他汀致脾控血不全出血斑马鱼模型的影响(中医理论

IF 2 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Journal of Traditional Chinese Medicine Pub Date : 2018-06-01 DOI:10.1016/S0254-6272(18)30630-7
Gao Chong , Wang Jun , Wang Jia , Zhu Xiaoyu , Zhu Changle , Guo Shengya , Wang Chong , Fan Qiuyue , Zhang Jianuo , Xia Bo , Chen Xinyi
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引用次数: 2

摘要

目的从中医理论角度探讨健脾益气阻血方对辛伐他汀所致出血、脾不控血证型斑马鱼模型的止血作用。方法实验1将60尾AB株野生型斑马鱼随机分为正常组和模型组。模型组给予50 μM辛伐他汀治疗24 h。第二实验:将黑色素等位基因突变的Albino斑马鱼分为正常组、模型组、A组和B组。观察参数为血流量、运动速度、出血率、出血改善率。结果脑出血率:第一次实验脑出血率为75%。在第二个实验中,心脏出血率为65%。血流量:与正常组比较,模型组大鼠血流量明显减少(P <0.001)。运动速度:第一次实验时,模型组与正常组比较,运动速度明显降低(P <0.001)。出血改善率:A剂对斑马鱼心脏出血效果不明显;B剂能降低斑马鱼心脏出血率,提高出血改善率。结论辛伐他汀所致出血斑马鱼模型的表现与脾控血失败所致的临床症状型基本相似。健脾益气阻血方可降低辛伐他汀所致斑马鱼心脏出血率,提高出血改善率。
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Effect of spleen-invigorating, Qi-replenishing and blood-arresting formula on zebrafish models with simvastatin-induced hemorrhage caused by spleen failing to control blood, in terms of theory of Traditional Chinese Medicine

OBJECTIVE

To investigate the hemostasis effect of spleen-invigorating, Qi-replenishing and blood-arresting formula, on a zebrafish models with simvastatin-induced hemorrhage, and with symptom pattern caused by spleen failing to control blood, in terms of theory of Traditional Chinese Medicine (TCM).

METHODS

In the first experiment, 60 AB strain wild type zebrafishes were randomly assigned into two groups: normal group and model group. The model group was treated with 50 μM simvastatin for 24 h. The second experiment: The melanin allele mutated Albino strain zebrafishes were divided into normal, model, A group and B group. The observational parameters were as follows: blood flow, velocity of movement, hemorrhage ratio and improvement ratio of hemorrhage.

RESULTS

Hemorrhage ratio: in the first experiment, brain hemorrhage ratio was 75%. In the second experiment, heart hemorrhage ratio was 65%. Blood flow: compared with the normal group, there was a significantly decrease in the model group (P < 0.001). Velocity of movement: in the first experimental, compared with the normal group, there was a significantly decrease in the model group (P < 0.001). Improvement ratio of hemorrhage: agents A had little effect in heart hemorrhage of the zebrafish; agents B could reduce heart hemorrhage ratio of the zebrafish, and increase the improvement ratio of hemorrhage.

CONCLUSION

The manifestation of zebrafish model with simvastatin-induced hemorrhage is basically similar to that of the clinical symptom pattern caused by spleen's failure to control blood. The Spleen-invigorating, Qi-replenishing and Blood-arresting Formula can reduce the heart hemorrhage ratio of zebrafish induced by simvastatin, and increase the Improvement ratio of hemorrhage.

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来源期刊
Journal of Traditional Chinese Medicine
Journal of Traditional Chinese Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
2.40
自引率
3.80%
发文量
32269
审稿时长
2 months
期刊介绍: Journal of Traditional Chinese Medicine(JTCM) is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.
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